基于微流控芯片对流感嗜血杆菌的快速检测

Rapid detection of Haemophilus influenza based on microfluidic chip

目的分析微流控芯片快速检测流感嗜血杆菌(HI)的优势。方法采用计算机辅助软件设计并制作微流控芯片,筛选最佳进样流速和进样时间,结合免疫荧光法进行HI捕获检测实验,并与平板培养法进行一致性对比。结果选择最佳进样流速(4 μL/min),进样30 min进行检测。建立的微流控芯片检测方法对HI的捕获率为95%,检测限为10^2 cfu/mL,特异性为100%,与其他几类常见呼吸道感染菌无交叉反应,检测过程可在30 min内完成,与平板培养法的总符合率为95%。结论微流控芯片检测方法对HI具有较高的捕获率,且具有简便、快速、特异性强的优点,对比目前临床先进的全自动快速微生物质谱检测系统,大大减少了检测成本,可适用于基层医疗单位及社区健康医疗服务中心。

Objective To analyze the advantages of a microfluidic chip for rapid detection of Haemophilus influenzae (HI).Methods The microfluidic chip was designed by computer-aided software and manufactured,and the best injection flow rate and injection time were optimized.The detection of HI was performed by immunofluorescence,and the results were compared with those by plate culture.Results The best injectionflow rate was selected as 4 μL/min,injecting for 30 minutes for detection.The established microfluidic chip for detection of HI had the capture efficiency of 95%,with the detection limit of 10^2 cfu/mL and specificity of 100%.There was no cross-reactivity with several other common respiratory infection bacteria,and the detection process can be completed within 30 minutes,with the total coincidence of 95% compared with plate culture method.Conclusions The microfluidic chip detection method has a high capture rate for HI,and has the advantages of simplicity,fastness and strong specificity.Compared with the current clinically advanced fully automatic fast microbial mass spectrometry detection system,it greatly reduces the cost,and is applicable to primary medical units and community health service centers.