PCR检测技术在真菌性角膜炎快速诊断中的应用

Application of PCR detection in rapid diagnosis of fungal keratitis

目的探讨聚合酶链反应(PCR)检测技术在真菌性角膜炎快速诊断中的应用。方法留取攀枝花学院附属医院502例疑似感染性角膜炎患者角膜分泌物分别进行革兰染色镜检、真菌培养和PCR检测,比较3种方法的检出率。结果革兰染色镜检、真菌培养、PCR检出率分别为5.6%(28例)、4.8%(24例)、5.9%(30例),三者比较,差异无统计学意义(χ~2=0.722,P=0.697)。结论 PCR检测、革兰染色镜检和真菌培养3种方法对真菌性角膜炎的检出率无明显差异,PCR检测具有能更快速地诊断真菌性角膜炎的特点。

Objective To explore the application of PCR detection in rapid diagnosis of fungal keratitis.Methods A total of 502 specimens of corneal secretions were taken from Affiliated Hospital of Panzhihua University by fungal smear,fungal culture and PCR detection.Then the results of the three methods were compared.Results In the 502 cases,28 positive cases were observed by fungal smear(5.6%),24 positive cases were observed by fungal culture(4.8%),30 positive cases were observed by PCR detection(5.9%),there was no significant statistical difference in three methods(χ~2=0.722,P=0.697).Conclusion There was no significant difference on the diagnosis of fungal keratitis among PCR detection,fungal smear and fungal culture.PCR detection is more rapidly for the diagnosis of fungal keratitis.