摘要
目的探讨新型冠状病毒(SARS-CoV-2)IgM和IgG抗体不同检测方法在新型冠状病毒肺炎(COVID-19)中的应用。方法选取25例COVID-19患者,以同期20例排除SARS-CoV-2感染的患者作为对照组,分别采用磁微粒化学发光法和胶体金法检测所有对象的血清SARS-CoV-2 IgM和IgG抗体。同时检测COVID-19患者血清降钙素原(PCT)、铁蛋白及C反应蛋白(CRP)。结果化学发光法检测血清SARSCoV-2 IgM和IgG抗体的敏感性分别为48%和56%,特异性均为100%,胶体金法检测血清SARS-CoV-2 IgM和IgG抗体的敏感性分别为88%和76%,临床特异性均为100%。2种方法检测血清SARS-CoV-2 IgM和IgG抗体总符合率分别为68.9%和73.3%。25例COVID-19患者中有36%的患者血清PCT升高、72%的患者血清CRP升高、84%的患者血清铁蛋白水平升高。结论SARS-CoV-2 IgM和IgG抗体不同检测方法之间差异较大,用于COVID-19患者的临床诊断时应综合考虑。
Objective To compare different detection methods of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)IgM and IgG antibodies in the diagnosis of corona virus disease 2019(COVID-19).Methods Serum samples were collected from 25 patients diagnosed as COVID-19,and 27 patients without SARS-CoV-2 infection were enrolled as control group.SARS-CoV-2 IgM and IgG antibodies were detected by chemiluminescence and colloidal gold method.The procalcitonin(PCT),C-reactive protein(CRP)and ferritin were also determined.Results The sensitivities of SARS-CoV-2 IgM and IgG antibodies by chemiluminescence were 48%and 56%,respectively,while those by colloidal gold method were 88%and 76%,respectively.The specificities were both 100%for the 2 methods.The total coincidence rates of IgM and IgG antibodies between the 2 methods were 68.9%and 73.3%,respectively.Serum PCT levels were increased in 36%COVID-19 patients,serum CRP levels were increased in 72%COVID-19 patients,and serum ferritin were increased in 84%COVID-19 patients.Conclusions The clinical performance of different detection methods of SARS-CoV-2 IgM and IgG antibodies are varied,which should be considered comprehensively in the clinical diagnosis of COVID-19.
作者
郑培明
崔发财
张福明
李刚
ZHENG Peiming;CUI Facai;ZHANG Fuming;LI Gang(Department of Clinical Laboratory,Henan Provincial People's Hospital,Zhengzhou University People's Hospital,Henan University People's Hospital,Zhengzhou 450003,Henan,China)
出处
《检验医学》
CAS
2020年第4期291-294,共4页
Laboratory Medicine
基金
国家自然科学基金青年项目(81802094)。
关键词
新型冠状病毒
免疫球蛋白
抗体
化学发光法
胶体金法
新型冠状病毒肺炎
Severe acute respiratory syndrome coronavirus 2
Immunoglobulin
Antibody
Chemiluminescence
Colloidal gold method
Corona virus disease 2019