GATA6和TRPC6在结肠癌组织中的表达及临床意义

Expression and clinical significance of GATA6 and TRPC6 in colon cancer

目的探讨转录因子GATA6和瞬时受体电位阳离子通道蛋白(TRPC)6在结肠癌组织中的表达及临床意义。方法选择河北省保定市第四中心医院和保定市第二医院行手术切除并经病理证实的结肠癌患者136例,免疫组化法及ELISA法测定GATA6和TRPC6在结肠癌组织及癌旁正常结肠组织中的蛋白表达情况与水平,分析GATA6、TRPC6表达水平与结肠癌患者临床病理特征及预后的关系。结果免疫组化结果显示,结肠癌组GATA6阳性102例,TRPC6阳性106例;结肠癌组GATA6阳性表达率(75.00%vs 13.24%,P<0.01)、TRPC6阳性表达率(77.94%vs 4.41%,P<0.01)高于癌旁组;结肠癌组GATA6、TRPC6蛋白表达评分高于癌旁组(P均<0.01)。ELISA法结果显示,结肠癌组GATA6、TRPC6蛋白表达水平高于癌旁组(P均<0.01)。GATA6、TRPC6蛋白表达与年龄关联性不明显(P均>0.05),与肿瘤最大径、病理学分级、TNM分期、浸润深度、淋巴血管间隙浸润、淋巴结转移、复发有明显关联性(P均<0.01)。GATA6阴性组(76.47%vs 29.41%,P<0.01)和TRPC6阴性组5年生存率(80.00%vs 30.19%,P<0.01)分别高于GATA6、TRPC6阳性组,生存期亦明显高于GATA6、TRPC6阳性组(P均<0.01)。结论GATA6、TRPC6蛋白在结肠癌组织中表达量增加,GATA6、TRPC6可能在结肠癌的发生发展过程中起促进作用,GATA6、TRPC6蛋白低表达的结肠癌患者可能获得较好的预后。

Objective To investigate the expression and clinical significance of transcription factor GATA6 and transient receptor potential cation channel protein(TRPC)6 in colon cancer tissues.Methods A total of 136 patients with colon cancer confirmed by pathology in the Fourth Central Hospital of Baoding and the Second Hospital of Baoding were selected.Immunohistochemistry and enzyme-linked immunosorbent assay(ELISA)were used to detect the expression levels of GATA6 and TRPC6 in colon cancer tissues(colon cancer group)and normal colon tissues(para-cancerous group)to analyze the associations of the expressions of GATA6 and TRPC6 with the clinicopathological characteristics and prognosis of patients with colon cancer.Results The immunohistochemical results showed that 102 cases were positive for GATA6,and 106 cases were positive for TRPC6 in colon cancer group;the positive rates of GATA6(75.00%vs 13.24%,P<0.01)and TRPC6(77.94%vs 4.41%,P<0.01)in colon cancer group were significantly higher than those in paracancerous group.The expression scores of GATA6 and TRPC6 in colon cancer group were statistically higher than those in para-cancerous group(all P<0.01).The expressions of GATA6 and TRPC6 protein were not significantly correlated with age(all P>0.05),but were significantly correlated with the largest diameter of tumor,pathological grade,TNM stage,infiltration depth,lympha-vascular space invasion,lymph node metastasis,and recurrence(all P<0.01).The 5-year survival rates in GATA6 negative group(76.47%vs 29.41%)and in TRPC6 negative group(80.00%vs 30.19%)were significantly higher than those in GATA6 and TRPC6 positive groups respectively,and the survival periods were also significantly higher than those in GATA6 and TRPC6 positive groups(all P<0.01).Conclusions The high expression levels of GATA6 and TRPC6 in colon cancer tissues may play a promoting role in the development of colon cancer.The patients with low expressions of GATA6 and TRPC6 may obtain a good prognosis.