Poly(VBA-co-VBTAC)/XF-70的制备及抑菌作用的研究

Preparation and Bacteriostasis Effects of Poly(VBA-co-VBTAC)/XF-70

目的:评估聚(4-乙烯基苯甲酸共(ar-乙烯基苄基))三甲基氯化铵Poly(VBA-co-VBTAC)/XF-70纳米纤维的理化性质,探讨其抑制细菌生物膜形成作用。方法:制备与Poly(VBA-co-VBTAC)/XF-70纳米纤维,扫描电镜(SEM)观察其微观形貌。使用高效液相(HPLC)测试释放曲线,建立金黄色葡萄球菌、牙龈卟啉单胞菌生物膜的体外模型。取1.00 mg/L的XF-70和相同XF-70含量的Poly(VBA-co-VBTAC)/XF-70进行抑菌试验。结果:XF-70在300 min内的释放量约为80%,XF-70组和Poly(VBA-co-VBTAC)/XF-70组细菌生物膜形成量显著降低,Poly(VBA-co-VBTAC)/XF-70的抑菌效果显著优于XF-70(P<0.05)。结论:Poly(VBA-co-VBTAC)/XF-70有良好的释放曲线,可显著提高XF-70对金黄色葡萄球菌、牙龈卟啉单胞菌生物膜形成的抑制作用。

Objective:To evaluate the physicochemical properties of poly(4-vinyl benzoic acid co(ar-vinyl benzyl))trimethylammonium chloride poly(VBA-co-VBTAC)/XF-70 nanofibers,and to explore their inhibitory effects on the formation of bacterial biofilms.Methods:Poly(VBA-co-VBTAC)nanofibers were prepared and compared with Poly(VBA-co-VBTAC)/XF-70.The morphology of the nanofibers was observed by SEM.The release curves of Staphylococcus aureus and Porphyromonas gingivalis biofilms were measured by high-performance liquid chromatography(HPLC),and the in vitro models of Staphylococcus aureus and Porphyromonas gingivalis biofilms were established.Bacterial inhibition tests were carried out on 1.00 mg/L XF-70 and Poly(VBA-co-VBTAC)/XF-70 with the same content of XF-70,respectively.Results:The release of XF-70 within 300 minutes was nearly 80%.The biofilm formation(absorbance value)of the two bacteria decreased significantly,and the bacteriostasis effect of Poly(VBA-co-VBTAC)/XF-70 was significantly better than that of XF-70 group(P<0.05).Conclusion:Poly(VBA-co-VBTAC)/XF-70 has a good short-term release curve,which can improve the inhibition of XF-70 on the formation of biofilm of Staphylococcus aureus and Porphyromonas gingivalis.