GATA3、MGB和GCDFP-15在乳腺癌中的病理诊断价值

Diagnostic value of GATA3,MGB and GCDFP-15 in breast cancer

目的探讨GATA结合蛋白3(GATA3)、乳腺珠蛋白(MGB)和巨囊性病液体蛋白-15(GCDFP-15)在乳腺癌中的病理诊断价值。方法采用免疫组化方法检测GATA3、MGB、GCDFP-15、雌激素受体(ER)、孕激素受体(PR)、人表皮生长因子受体2(HER2)、Ki67在乳腺癌中的表达,并根据组织来源及免疫表型将乳腺癌分为原发性/转移性乳腺癌、激素受体阳性/阴性乳腺癌及不同分子分型的乳腺癌,统计GATA3、MGB、GCDFP-15阳性率。结果GATA3在原发性/转移性乳腺癌中的阳性率(92.5%;94.25%)均显著高于MGB(42.11%;29.17%)和GCDFP-15(55.77%;31.34%)(P<0.01),对于评估原发灶不明来源的肿瘤,尤其是恶性积液的标本,GATA3具有更好的敏感性。此外,MGB和GCDFP-15在激素受体阴性和三阴型乳腺癌中的阳性率偏低,进一步加大了这两种转移性乳腺癌的诊断难度。而GATA3在激素受体阴性(90.38%)或三阴型乳腺癌中的阳性率(85.29%)显著高于MGB(20.00%;5.00%)和GCDFP-15(35.09%;33.33%)(P<0.01),提示其在激素受体阴性和三阴型转移性乳腺癌中具有潜在诊断价值,是一个优于MGB和GCDFP-15的特异性标记物。结论对于评估原发灶不明来源的肿瘤,尤其是恶性积液的标本,或免疫表型为激素受体阴性和三阴性的乳腺癌,GATA3具有比MGB和GCDFP-15更高的诊断价值。

Objective To investigate the diagnostic value of GATA binding protein 3(GATA3),mammaglobin(MGB)and gross cystic disease fluid protein-15(GCDFP-15)in breast cancer.Methods Immunohistochemistry was performed to detect the expression of GATA3,MGB,GCDFP-15,estrogen receptor(ER),progesterone receptor(PR),human epidermal growth factor receptor 2(HER2)and Ki-67 in breast cancer.The breast cancer was divided into primary/metastatic breast cancer,hormone receptor positive/negative,or different molecular types of breast cancer according to the tissue origin and immunophenotype.The positive rates of GATA3,MGB and GCDFP-15 were statistically analyzed.Results The positive rate of GATA3 in primary/metastatic breast cancer(92.5%;94.25%)was significantly higher than that of MGB(42.11%;29.17%)and GCDFP-15(55.77%;31.34%)(P<0.01),and GATA3 had better sensitivity for evaluating the tumor of unknown origin,especially in malignant hydrops.In addition,the positive rate of MGB and GCDFP-15 in hormone receptor negative and triple negative breast cancer was low,which further increased the difficulty of diagnosis of these two kinds of metastatic breast cancer.However,the positive rate of GATA3 in hormone receptor negative(90.38%)or triple negative breast cancer(85.29%)was significantly higher than that of MGB(20.00%;5.00%)and GCDFP-15(35.09%;33.33%)(P<0.01),suggesting that it had great potential diagnostic value in hormone receptor negative and triple negative metastatic breast cancer and was a specific marker superior to MGB and GCDFP-15.Conclusion GATA3 has a higher diagnostic value than MGB and GCDFP-15 for the assessment of tumors of unknown origin,especially in malignant hydrops,or breast cancer with an immunophenotype of hormone receptor negative and triple negative breast cancer.