乳腺癌circ0008234和miR-1205表达与CT征象的相关性分析

Comparative analysis of oncology features of CT signs and expression of circ0008234 and miR-1205 in breast cancer

目的探讨乳腺癌circ0008234和miR-1205表达与多层螺旋CT显像的肿瘤学特点。方法选取2018年1月至2018年12月浙江台州市第一人民医院的40例乳腺癌患者作为观察组,将同期收治的40例非乳腺癌患者作为对照组,荧光定量PCR法检测所有患者circ0008234和miR-1205表达水平。通过生物信息学方法预测circ0008234的下游靶miRNA,双荧光素酶报告分析法证实circ0008234和miR-1205的相互作用。采用多层螺旋CT对患者进行检查,观察肿块大小、形态、钙化区域、淋巴结转移及边缘,分析circ0008234和miR-1205表达水平与CT征象之间的相关性。结果观察组circ0008234表达水平高于对照组(2.23±0.86 vs 1.07±0.37)(P=0.00),但miR-1205表达水平则低于对照组(0.76±0.29 vs 1.04±0.29)(P=0.00)。观察组患者circ0008234和miR-1205表达水平在不同边缘形态及微小钙化点患者体内无显著差异,但肿块形态规则的患者circ0008234表达水平显著高于不规则患者(2.52±0.88 vs 1.91±0.74)(P=0.025),miR-1205表达水平则低于不规则患者(0.66±0.30 vs 0.86±0.25)(P=0.025)。观察组肿块直径≥2 cm患者circ0008234表达水平显著高于肿块直径<2 cm患者(2.59±0.95 vs 1.69±0.17)(P=0.001),但miR-1205表达水平则低于肿块直径<2 cm患者(0.65±0.21 vs 0.92±0.33)(P=0.003)。观察组淋巴结转移患者circ0008234表达水平显著高于无淋巴结转移患者(2.55±1.09 vs 1.94±0.44)(P=0.022),但淋巴结转移患者miR-1205表达水平低于无淋巴结转移患者(0.65±0.26 vs 0.85±0.30)(P=0.027)。荧光素酶检测证实miR-1205是circ0008234的直接靶点。circ0008234可负调控miR-1205的表达。结论乳腺癌CT显像与circ0008234和miR-1205表达间存在相关性,这可为乳腺癌恶变程度和预后的判断提供依据。

Objective To investigate the expression of circ0008234 and miR-1205 in breast cancer and the oncology characteristics of CT.Methods 40 patients with breast cancer were collected as observational objects from Jan.2018 to Dec.2018 and 40 patients without breast cancer were selected as the control group.The expression levels of circ0008234 and miR-1205 of all patients were estimated by quantitative real-time polymerase chain reaction.Bioinformatics was used for the predictions of circ0008234 target miRNA.A dual-luciferase reporter assay was used to confirm the interaction between circ0008234 and miR-1205.All patients of the observation group were examined by multi-slice CT.CT images were analyzed through observing tumor size,shape,calcification area,lymph node metastasis and margin.The correlation between CT signs and the expression of circ0008234 and miR-1205 was further analyzed.Results The levels of circ0008234 of observation group were significantly higher than those of the control group(2.23±0.86,1.07±0.37,P=0.00),but the expression levels of miR-1205 were lower than the control group(0.76±0.29,1.04±0.29,P=0.00).In the observation group,there were no significant differences in the expression of circ0008234 and miR-1205 among patients with different tumor marginal morphology and micro calcifications.However the expression of circ0008234 in patients with regular lump form were significantly higher than those in patients with inregular form(2.52±0.88,1.91±0.74,P=0.025),and the expression of miR-1205 were lower than those in patients with inregular form(0.66±0.30,0.86±0.25,P=0.025).In the observation group,the expression of circ0008234 in patients with mass diameter≥2 cm were significantly higher than those in patients with mass diameter<2 cm(2.59±0.95,1.69±0.17,P=0.001),but the expression of miR-1205 were lower than those in patients with mass diameter<2 cm(0.65±0.21,0.92±0.33,P=0.003).In the observation group,the expression of circ0008234 in patients with lymph node metastasis were higher than those without lymph node metastasis(2.55±1.09,1.94±0.44,P=0.022),but the expression of miR-1205 in patients with lymph node metastasis were lower than those without lymph node metastasis(0.65±0.26,0.85±0.30,P=0.027).miR-1205 was verified as a direct target of circ0008234 by luciferase assay.circ0008234 could negatively regulate the expression of miR-1205.Conclusion There is a correlation between CT imaging signs and the expression of circ0008234 and miR-1205 in patients with breast cancer,which can provide more reference for the judgment of malignant degree and prognosis of patients with breast cancer.