中频交变微电流联合紫杉醇抗A549细胞作用的研究

Effect of Alternating Micro-current at Intermediate Frequency(ACIF)Combined with Paclitaxel on A549 Cells

目的:研究中频交变微电流联合紫杉醇注射液抗A549细胞的作用及机制。方法:对处于对数生长期的肺腺癌A549细胞施加电刺激、紫杉醇及电刺激联合紫杉醇三种不同处理,采用MTT法检测A549细胞存活率,并利用流式细胞仪测量分析各组细胞凋亡/死亡比例及细胞周期状态。结果:通过不同参数的中频交变微电流刺激A549细胞,得到的最低细胞存活率(参数150 kHz、90 m A、30 min)为78.02±0.73%(P<0.01);联合紫杉醇注射液半抑制浓度(IC50)干预后,细胞存活率为32.87±0.94%(P<0.01);同时发现中频交变微电流联合紫杉醇注射液能促进A549细胞凋亡,阻滞细胞于S期、G2/M期。结论:中频交变微电流可抑制A549细胞增殖、促进凋亡,但对细胞周期影响不明显;与紫杉醇注射液联合应用时具有协同增强抗肿瘤作用。

Objective:To study the effect and mechanism of Alternating Micro-current at Intermediate Frequency(ACIF)in combination with paclitaxel injection against A549 cells in vitro.Methods:The A549 cells was exposed to ACIF,paclitaxel and the combination of both in logarithmic phase.The cell growth rate was measured by MTT assay.Cell Apoptosis/death rate and cell cycle status were analyzed by flow cytometry.Results:A549 cells were exposed to different ACIF parameters.The lowest cell survival rate was78.02±0.73%(parameter 150 kHz,90 m A,30 min)(P<0.01).After the intervention of paclitaxel(IC50),the cell survival rate was32.87±0.94%(P<0.01).It was found that the combination of ACIF and paclitaxel can promote apoptosis of A549 cells,and block cells in S and G2/M phases.Conclusion:ACIF can promote apoptosis of A549 cells,but has no obvious effect on cell cycle.When combined with paclitaxel injection,it has synergistic enhancement of anti-tumor effect.