食管鳞状细胞癌中SSBP1和mtTFA的表达及其临床病理意义

Expression and Clinicopathological Significances of SSBP1 and mtTFA in the Esophageal Squamous Cell Carcinoma

目的:探讨食管鳞状细胞癌(Esophageal squamous cell carcinoma,ESCC)组织中线粒体单链DNA结合蛋白(single-strand DNA-binding protein 1,SSBP1)与线粒体转录因子A(mitochondrial transcription factor A,mtTFA)的表达及其临床病理意义。方法:采用免疫组化染色技术检测40例食管鳞状细胞癌组织与20例正常食管组织中SSBP1与mtTFA蛋白的表达,并分析其与食管鳞状细胞癌临床病理特征的相关性。结果:食管鳞状细胞癌组织中SSBP1和mtTFA的表达阳性率均明显高于正常食管组织(x^2=9.909,P=0.002;x^2=23.177,P=0.000)。SSBP1、mtTFA蛋白的表达与食管鳞状细胞癌的临床分期(x^2=6.506,P=0.039;x^2=7.535,P=0.023)、淋巴结转移(x^2=7.305,P=0.007;x^2=4.815,P=0.028)显著相关;mtTFA蛋白的表达与食管鳞状细胞癌的浸润深度有关(x^2=8.541,P=0.014),SSBP1的表达与浸润深度无关(x^2=5.695,P=0.058)。食管鳞状细胞癌组织中SSBP1与mtTFA的表达呈正相关(r=0.392,P=0.012)。结论:食管鳞状细胞癌组织中SSBP1和mtTFA蛋白均呈表达上调,二者可能协同作用参与食管鳞癌的发生、发展。

Objective:To investigate the expression and clinicopathological significances of mitochondrial DNA binding protein 1(SSBP1)and mitochondrial transcription factor A(mtTFA)in the esophageal squamous cell carcinoma(ESCC)tissues.Methods:Immunohistochemical staining was used to detect the expression of SSBP1 and mt-TFA protein in 40 cases of esophageal squamous cell carcinoma and 20 normal esophageal tissue.Their correlation with the clinicopathological features of ESCC were analyzed.Results:The positive rates of SSBP1 and mtTFA in the ESCC were significantly higher than those in the normal esophageal tissues(x^2=9.909,P=0.002;x^2=23.177,P=0.000).The expression of SSBP1,mtTFA protein were significantly related to the clinical stage of esophageal squamous cell carcinoma(x^2=6.506,P=0.039;x^2=7.535,P=0.023),lymph node metastasis(x^2=7.305,P=0.007;x^2=4.815,P=0.028).The expression of mtTFA protein was related to the depth of invasion of esophageal squamous cell carcinoma(x^2=8.541,P=0.014).The expression of SSBP1 showed no relationship with the depth of invasion(x^2=5.695,P=0.058).There was a positive correlation between SSBP1 and mtTFA expression in esophageal squamous cell carcinoma(r=0.392,P=0.012).Conclusions:SSBP1 and mtTFA proteins are up-regulated in the esophageal squamous cell carcinoma,and they may participate in the development and progression of esophageal squamous cell carcinoma.