RNA甲基化酶NSUN2对胃癌细胞侵袭和迁移的影响及机制研究

Inhibiting RNA methylase NSUN2 reduce the invasion and migration of gastric cancer cell

目的:研究RNA甲基化酶NSUN2对胃癌细胞侵袭以及迁移能力的影响,并探讨其机制。方法:抑制NSUN2基因的慢转染病毒和阴性对照的空载病毒分别转染进胃癌SGC7901细胞和MGC803细胞,建立干扰组和对照组。实时荧光定量PCR法和Western blot法检测两组NSUN2的m RNA和蛋白表达;Transwell和划痕实验研究NSUN2对细胞迁移和侵袭的影响;Western blot法检测两组细胞中上皮细胞-间充质转化(EMT)分子标志物(E-Cadherin、N-Cadherin)蛋白表达情况。结果:与对照组相比,干扰组细胞RNA甲基化酶NSUN2 m RNA和蛋白表达量下降,穿膜细胞数减少,迁移距离缩短,N-Cadherin蛋白表达量降低,E-Cadherin蛋白表达量升高,差异均具有统计学意义(P<0.05)。结论:抑制NSUN2基因表达可降低胃癌细胞SGC7901和MGC803的侵袭和迁移,其机制是抑制EMT过程。

AIM:To investigate the effect of RNA methylase NSUN2 on the invasion and migration ability of gastric cancer cells,and to explore its mechanism.METHODS:The slow-transfected virus that inhibited the NSUN2 gene and the no-load virus that was negative control were transfected into gastric cancer SGC7901 cell lines and MGC803 cell lines,respectively.Real-time quantitative PCR and Western blot were used to detect the m RNA and protein expression of NSUN2 in two groups,TRANSWELL and scratch experiments were used to study the effects of NSUN2 on cell migration and invasion,Western blot was used to detect the Epithelial-Mesenchymal Transition(EMT)molecules in the two groups of cells Marker(E-Cadherin,N-Cadherin)protein expression.RESULTS:Compared with the control group,the expression of RNA methylase NSUN2 m RNA and protein in the interference group decreased,the number of transmembrane cells decreased,the migration distance decreased,the expression of N-Cadherin protein decreased,and the expression of E-Cadherin protein increased.All differences have statistical significance.CONCLUSION:Inhibition of NSUN2 gene expression can reduce the invasion and migration of gastric cancer cell lines SGC7901 and MGC803,and the mechanism is to inhibit the EMT process.