梅里埃MALDI-TOF-MS系统与VITEK 2 Compact全自动微生物鉴定仪对临床常见病原菌鉴定的一致性分析

Consistency Analyzer for Identification of Common Clinical Pathogenic Bacteria by the Merrier MALDI-TOF-MS System and VITEK 2 Compact

目的分析梅里埃基质辅助激光解吸电离飞行时间质谱(matrix-assisted laser desorption ionization-time offlight mass spectrometry,MALDI-TOF-MS)系统与VITEK 2 Compact全自动微生物鉴定仪对临床常见病原菌鉴定的一致性。方法收集福建医科大学孟超肝胆医院2018年6月~2019年3月来自临床不同标本分离的非重复菌株300株,分别采用梅里埃MALDI-TOF-MS系统和VITEK 2 Compact全自动微生物鉴定仪对病原菌进行鉴定,结果不一致菌株采用16SrDNA基因测序予以确认,比较两种鉴定方法的准确性与一致性,评估两种方法在鉴定16株大肠埃希菌的时间和经济成本。结果 300株临床分离菌中,鉴定到种水平的菌株MALDI-TOF-MS有268株(89.33%),VITEK 2 Compact有266株(88.67%);鉴定到属水平的菌株MALDI-TOF-MS有297株(99.00%),VITEK 2 Compact有297株(99.00%);两种方法在种属鉴定的一致性分别为82.67%与98.33%,MALDI-TOF-MS的准确性略高于VITEK 2 Compact。两种方法鉴定不一致菌株有39株(13.00%),与16SrDNA测序鉴定结果相比,MALDI-TOF MS的准确率(79.49%)高于VITEK 2Compact(58.97%)。对16株大肠埃希菌的鉴定,MALDI-TOF-MS耗时较VITEK 2 Compact少3.5h,且成本仅为VITEK2 Compact的2/5。结论 MALDI-TOF MS与VITEK 2 Compact对于临床常见病原菌的鉴定结果较为一致,但MALDITOF MS准确率更高,且更快速与经济。

Objective To analyze the consistency of matrix-assisted laser desorption mass-time of flight mass spectrometry(MALDI-TOF-MS) system and VITEK 2 Compact automatic microbial analyzer in the identification of common pathogens.Methods A total of 300 non-repetitive isolates were collected from clinical culture samples in Meng chao Hepatobiliary Hospital of Fujian Medical University from June 2018 to Mar 2019,and identified by MALDI-TOF-MS and VITEK 2 Compact system,respectively. The conflicting results between them were further verified by 16 SrDNA gene sequencing. The accuracy and consistency of the two identification methods were compared, and the time and economic cost of identifying 16 strains of Escherichia coli by the two methods were evaluated. Results Among the 300 strains, 268 strains(89.33%) of MALDI-TOFMS and 266 strains(88.67%) of VITEK 2 Compact were identified. 297 strains(99.00%) of MALDI-TOF-MS and 297 strains(99.00%) of VITEK 2 Compact were identified. The consistency of the two methods in species identification was 82.67% and 98.33%, respectively. The accuracy of MALDI-TOF-MS was slightly higher than that of VITEK 2 Compact. There were 39 strains(13.00%) identified by the two methods, and the accuracy of MALDI-TOF-MS(79.49%) was higher than that of VITEK 2 Compact(58.97%) compared with the 16 SrDNA sequencing results. The identification of 16 strains of Escherichia coli showed that the time of MALDI-TOF-MS was 3.5 hours less than that of VITEK 2 Compact, and the cost was only 2/5 of VITEK 2 Compact. Conclusion The identification results of MALDI-TOF-MS and VITEK 2 Compact for common clinical pathogens were consistent, but the accuracy of MALDI-TOF-MS is higher, faster and more economical.