不同溶剂同时蒸馏萃取艾叶挥发油的抑菌活性

Antimicrobial Activities of Artemisia argyi Essential Oils Extracted by Simultaneous Distillation using Different Solvent Systems

采用二氯甲烷和正己烷2种溶剂,分别进行同时蒸馏萃取(SDE)工艺萃取艾叶挥发油,对得物进行气质联用(GC-MS)成分分析,测定其抑菌活性的差异,并通过扫描电镜(SEM)探究艾叶挥发油抑菌作用的机理。结果表明,SDE比水蒸气蒸馏萃取节省时间1 h,提取率达1.5%,是后者的3倍。SDE(正己烷)的得物抑菌活性更强,最低抑菌浓度集中在6.25μL/mL,GC-MS结果可知其成分的92.95%是水蒸气蒸馏得物和SDE(二氯甲烷)的公共主要成分,SEM结果显示艾叶挥发油可作用于微生物细胞壁结构,从而达到抑制效果。

In this study,Artemisia argyi essential oils were extracted by simultaneous distillation(SDE)with dichloromethane and n-hexane,respectively.To explore the differences of antimicrobial activities,components of the oil were analyzed by GC-MS,and scanning electron microscope(SEM)was used to explore the mechanism of antimicrobial activity.Compared with the steam distillation,SDE saved one hour and had a three times higher yield(1.5%).SDE(n-hexane)oil had a higher antimicrobial activity with the minimum inhibitory concentration(MIC)being 6.25μL/mL.GC-MS results revealed that the SDE(n-hexane)oil had 92.95%similarity in the chemical composition with the oils extracted with steam distillation and SDE(dichloromethane).SEM results showed that the antibacterial activity of Artemisia argyi essential oil could be achieved by disruption of cell wall.