MMP-2、MMP-9在恶性黑色素瘤中的表达及预后意义

Expression and Prognostic Significance of MMP-2 and MMP-9 in Malignant Melanoma

目的观察基质金属蛋白酶(MMP)-2、MMP-9在恶性黑色素瘤(Malignant melanoma,MM)细胞中的表达以及预后意义。方法采用免疫组化SP法分别检测52例MM组织、20例色素痣中MMP-2、MMP-9的表达,用χ^2检验分析MM组织与色素痣组织中二者表达的差异性,用多变量Cox回归分析其预后关系,单因素Kaplan-Meier法和对数秩检验进行生存分析。结果MM组织中MMP-2(90.4%)、MMP-9(78.8%)表达均显著高于色素痣组织(P<0.05);MMP-2、MMP-9表达阳性患者死亡风险显著升高;MMP-2阳性表达的MM患者的生存期显著低于阴性者(均P=0.004),MMP-9阳性表达的MM患者的生存期显著低于阴性者(均P<0.001)。结论MMP-2及MMP-9在MM组织中高表达,且与患者预后关系密切。

Objective In order to observe the expression of matrix metalloproteinase-2(MMP-2)and matrix metalloproteinase-9(MMP-9)in malignant melanoma cells and its prognostic significance.Methods Immunohistochemical SP method was used to detect the expression of MMP-2 and MMP-9 in 52 cases of malignant melanoma and 20 cases of pigmented nevus.The differences of expression between malignant tissue and pigmented nevus was analyzed by Chi-square test.Sexuality was analyzed by multivariate Cox regression.Results The expressions of MMP-2(90.4%)and MMP-9(78.8%)in malignant melanoma tissues were significantly higher than those in pigmented nevus tissues(P<0.05).The risk of death was significantly increased in patients with positive MMP-2 and MMP-9 expression.Patients with malignant melanoma with MMP-2 positive expression had significantly lower survival time than those with negative(log-rank P=0.004),and patients with malignant melanoma with MMP-9 positive expression had significantly lower survival time than those with negative(log-rank P<0.001).Conclusion MMP-2 and MMP-9 are highly expressed in malignant melanoma tissues and are closely related to the prognosis of patients.