肉鸡盲肠微生物群落特征与体质量的相关性

Correlration Analysis of Cecal Microbiota and Body Mass of Broilers

为探究盲肠微生物与肉鸡个体长势的相关性,将10周龄宁都黄鸡按体质量高低分为4组,利用高通量测序技术分析比较相同日龄、不同体质量笼养肉鸡的肠道微生物多样性及菌群结构特征。结果表明,4组样品获得的OTU数量及各组Alpha多样性指数Chaol值、Shannon和Simpson指数无显著差异;组间盲肠微生物群落组成具有相似性,但菌群相对丰度有显著差异;与肉鸡体质量呈正相关的菌群中,影响最大的是甲烷短杆菌属,其次是栖粪杆菌属,负相关菌群主要是脱硫弧菌属和互养菌属。研究显示肠道微生物群落结构与肉鸡个体长势显著相关,显著差异菌群可应用于改善肉鸡生长性能。

In order to analyze the correlation between cecal microbiota and growth performance of broilers,ten-week-old Ningdu Huang chickens were selected and divided into 4 groups according to the body mass from the high to the low.High-throughput sequencing was performed to analyze and compare the diversity and structure of intestinal microbes in cage broilers at the same age with different body mass.The results showed that there were no significant differences in OTU numbers and Alpha diversity indexes such as Chaol value,Shannon index and Simpson index among four groups with different body mass.The composition of cecal microbiota was similar among the different mass groups of Ningdu Huang chickens,while the relative abundance of bacterial communities was significantly different.The most influential biomarks positively related to the body mass was Methanobrevibacter,followed by Faecalibacterium,and the negatively related biomarks were Desulfovibrio and Synergistes.This study revealed that the structure of intestinal microbiota was significantly related to the growth performance of broilers.The biomarks identification could be applied to improve broiler growth performance.