基于^18F-APN-1607PET影像的阿尔茨海默病tau蛋白沉积相关疾病模式探究

Study on tau related disease pattern of Alzheimer′s disease based on ^18F-APN-1607 PET imaging

目的 探究阿尔茨海默病(AD)患者^18F-APN-1607 PET影像脑内tau蛋白沉积相关疾病模式(tauRDP).方法 收集复旦大学附属华山医院17例AD患者[男6例,女11例;年龄(61.7±12.3)岁;简易精神状态检查量表(MMSE)评分(17.6±7.9)分]和10名年龄性别匹配的健康对照(NC)者[男6名,女4名;年龄(61.2±4.7)岁]的^18F-APN-1607脑影像.在体素层面使用基于主成分分析(PCA)的尺度子轮廓模型(SSM)技术构建tauRDP,计算每个样本tauRDP表达值,通过两样本t检验比较tauRDP表达值在AD患者和健康对照者间的差异;使用Pearson相关分析AD患者tauRDP表达值与MMSE评分的相关性.结果 AD患者的tauRDP脑区主要包括:中央前回、背外侧额上回、额中回、岛盖部额下回、三角部额下回、补充运动区、内侧额上回、左侧内侧和旁扣带脑回、右侧楔叶、枕上回、枕中回、中央后回、顶上回、顶下缘角回、缘上回、角回、楔前叶和颞中回.AD患者tauRDP表达值为(12.6±8.0),与NC(0.0±1.0)间差异有统计学意义(t=4.395,P<0.001),且AD组tauRDP表达值与MMSE得分相关(r=-0.566,P=0.018).结论 基于SSM/PCA方法建立的tauRDP可用于定量表达tau蛋白沉积空间分布异常,tauRDP表达值有潜力用于评估AD严重程度.

Objective Exploring tau related disease pattern(tauRDP)in the brain of Alzheimer′s disease(AD)patients based on ^18F-APN-1607 PET scan.Methods ^18F-APN-1607 PET images were collected from 17 AD patients(6 males and 11 females,age:(61.7±12.3)years,Mini-Mental State Examination(MMSE)score:17.6±7.9)and 10 normal controls(NC;6 males and 4 females,age:(61.2±4.7)years)from Huashan Hospital of Fudan University.The scaled subprofile model(SSM)based on principal component analysis(PCA)technique was used to construct the tauRDP.Then the expression value of tauRDP in each sample was calculated.The differences on tauRDP expression values between AD patients and NC were compared by independent-sample t test.Pearson correlation analysis was used to analyze the correlation between tauRDP expression values and MMSE values in AD patients.Results The tauRDP area mainly included:precentral gyrus,dorsolateral superior frontal gyrus,middle frontal gyrus,inferior frontal gyrus of opercular part,inferior frontal gyrus of triangular part,supplementary motor area,medial superior frontal gyrus,left median cingulate and paracingulate gyri,right cuneus,superior occipital gyrus,middle occipital gyrus,postcentral gyrus,superior parietal gyrus inferior parietal,but supramarginal and angular gyri,supramarginal gyrus,angular gyrus,precuneus and middle temporal gyrus.There were significant differences(t=4.395,P<0.001)between AD group(12.6±8.0)and NC group(0.0±1.0)in tauRDP expression values.The tauRDP expression values were correlated with MMSE values in AD group significantly(r=-0.566,P=0.018).Conclusions TauRDP established basing on SSM/PCA method can be used to quantitatively express the abnormal spatial distributions of tau deposition.Expression value of tauRDP has the potential to initially assess the severity of AD.