P物质前体与血小板活化及血小板参数的相关性

Correlation between substance P precursors and platelet activation,platelet parameters

目的探讨P物质前体(Pro-SP)与血小板活化及血小板参数的相关性。方法总共纳入心内科患者76例,根据急性心肌梗死诊断标准将患者分为非急性心肌梗死组(39例)和急性心肌梗死组(38例)。采用流式细胞仪测定血小板-单核细胞聚集体(PMA)形成率评估血小板活化程度,酶联免疫吸附实验测定Pro-SP浓度。比较Pro-SP与PMA形成率及血小板参数相关性,以及两组间Pro-SP差异。结果Pro-SP与PMA形成率(Pearson相关系数为0.407,P<0.001)、血小板分布宽度(Pearson相关系数为0.269,P=0.018)呈正相关,与血小板总数、血小板平均容积、血小板比积、大血小板比例不相关(P>0.05)。多元线性回归分析显示Pro-SP是PMA形成率的独立影响因素,急性心肌梗死组Pro-SP较非急性心肌梗死组显著升高(P<0.05)。结论Pro-SP与血小板活化正相关,且与血小板分布宽度呈正相关,与血小板总数、血小板平均容积、血小板比积、大血小板比例不相关(P>0.05)。

Aim To discuss the correlation between substance P precursor(Pro-SP)and platelet activation,platelet parameters.Methods A total of 76 patients were enrolled in the department of cardiology.According to the diagnostic criteria of acute myocardial infarction,the patients were divided into non-acute myocardial infarction group(n=39)and acute myocardial infarction group(n=38).Percentage of platelet-mononuclear aggregate(PMA)formation was evaluated for platelet activation by flow cytometry.The concentration of Pro-SP was determined by ELISA.The correlations of Pro-SP,PMA and the parameters of platelets were statistically compared,as well as the differences between the two groups in Pro-SP.Results Pro-SP was positively correlated with PMA(Pearson correlation coefficient 0.407,P<0.001)and platelet distribution width(Pearson correlation coefficient 0.269,P=0.018),but not with total platelet count,mean platelet volume,platelet specific volume,and large platelet ratio(P>0.05).Linear regression analysis showed that Pro-SP was an independent influencing factor of PMA,and Pro-SP was significantly higher in the acute myocardial infarction group than that in the non-acute myocardial infarction group(P<0.05).Conclusion Pro-SP is positively correlated with platelet activation and platelet distribution width,but not with total platelet count,average platelet volume,platelet specific volume,and large platelet ratio(P>0.05).