超高效液相色谱质谱联用法检测水稻中双草醚的残留量

Analysis of Bispyribac-sodium Residue in Rice by UPLC-MS/MS

建立了基于UPLC-MS/MS的测定水稻中双草醚残留量的方法。用乙腈和水的混合物提取样品,进行纯化和检测。采用外部标准校准曲线定量分析。结果表明,双草醚在糙米、稻壳和稻秆中平均添加回收率分别为95%~101%、97%~101%和99%~113%,相对标准偏差分别为6%~8%、1%~4%和2%~3%。双草醚在糙米中的最低检出质量分数为0.02 mg/kg。该方法快速简便,准确可靠。

The method for determining bispyribac-sodium residue in rice was established based on UPLC-MS/MS.The sample was extracted with acetonitrile and water mixture,purified and detected.The quantitative analysis of external standard calibration curves was used.Results showed that the average recoveries of bispyribac-sodium in rice、rice husk and rice straw were 95%~101%,97%~101% and 99%~113%,the relative standard deviations were 6%~8%,1%~4% and 2%~3%.The limit of quantification for bispyribac-sodium in rice was 0.02 mg/kg.The method is fast,simple,accurate and reliable.