竹叶提取物对肉鸡早期血液生化和机体抗氧化功能的影响

Effects of Bamboo Leaf Extracts on Blood Biochemistry and Antioxidant Function in Broilers at Early Age

本研究旨在探讨日粮中添加竹叶提取物(Bamboo leaf extract,BLE)对肉鸡早期血液生化和机体抗氧化功能的影响,以期改善肉鸡健康,为人类提供安全放心肉产品。选用1日龄爱拔益佳(Arbor Acres,AA)雄性肉鸡256只随机分成4组,每组8个重复,每个重复8只鸡。对照组饲喂基础日粮,样品组分别在基础日粮中添加0.10%、0.20%和0.40%竹叶提取物,试验周期21 d。结果表明:与对照组相比,日粮添加竹叶提取物可降低血清中总胆固醇含量,其中0.20%竹叶提取物组降低效果显著(P<0.05),并且可显著降低血清中尿素氮含量(P<0.05);日粮添加竹叶提取物对肉鸡胸肌抗氧化酶活无显著影响,但可促进胸肌谷胱甘肽的生成。竹叶提取物可促进肝脏谷胱甘肽合成,其中0.20%和0.40%组肝脏谷胱甘肽含量显著高于对照组(P<0.05);此外,与对照组相比,日粮添加竹叶提取物对肝脏超氧化物歧化酶、谷胱甘肽过氧化物酶的活性具有一定的提高作用;与对照组相比,0.10%和0.20%组肉鸡血清中谷胱甘肽过氧化物酶活性分别提高5.82%和4.90%,其中0.10%竹叶提取物组显著高于对照组(P<0.05)。此外,与对照组相比,0.10%、0.20%和0.40%竹叶提取物组血清丙二醛含量分别降低了11.18%、14.59%和4.05%。综上,肉鸡早期日粮中添加一定剂量竹叶提取物未对肉鸡代谢造成影响,可提高机体抗氧化能力,从一定程度上达到改善肉鸡健康作用,可作为食品抗氧化剂使用。

This study was conducted to investigate the effects of dietary Bamboo Leaf Extract(BLE)on blood biochemistry parameters and antioxidant status in broiler chickens at early age,with purpose to improve the status of broilers and provide safe meat for human diet.A total of 2561-day-old male Arbor Acres broilers were randomly divided into 4 groups with 8 replicate each.A CON was fed basal diet,while three experimental groups were fed basal diet supplemented with 0.10%,0.20%and 0.40%BLE for 21 d,respectively.The results showed that supplemented with 0.20%BLE significantly reduced total cholesterol and urea nitrogen contents in serum as compared with CON(P<0.05).Dietary of BLE had no significant effect on the antioxidant capacity of breast meat in broiler chickens,but improved glutathione concentration.The liver glutathione contents in 0.20%and 0.40%BLE groups were significantly higher than CON group(P<0.05),in addition,BLE improved liver superoxide dismutase and glutathione peroxidase enzymes activity.Compared with CON group,the serum glutathione peroxidase activity in broiler chickens was increased by 5.82%and 4.90%in the 0.10%and 0.20%BLE groups,respectively,among which the 0.10%BLE group was significantly higher than the CON group(P<0.05).In addition,the serum malondialdehyde contents in 0.10%,0.20%and 0.40%BLE groups were reduced by 11.18%,14.59%and 4.05%respectively as compared with CON group.In conclusion,adding a certain amount of BLE into broilers’diets at early age without negative influence and improved health status and antioxidant capacity in broilers,which implied that Bamboo leaf extract act as food antioxidant had a certain feasibility.