循环间歇性低氧对大鼠肾上腺β淀粉样前体蛋白裂解酶1表达的影响

Effect of cyclic intermittent hypoxia on the expression ofβ-site amyloid precursor protein cleaving enzyme 1 in rat adrenal gland

目的观察β淀粉样前体蛋白裂解酶1(BACE1)在大鼠肾上腺的表达和定位,检测循环间歇性低氧(CIH)对BACE1表达的影响。方法采用Western blotting和免疫组织化学法检测BACE1在大鼠肾上腺的表达及定位;16只雄性SD大鼠随机分为2组,常氧对照组(control组)和CIH模型组,每组8只。模型制作2周,Western blotting检测各组大鼠肾上腺髓质BACE1和酪氨酸羟化酶(TH)蛋白的表达量。结果BACE1主要定位于大鼠肾上腺髓质神经纤维;与control组相比,CIH组大鼠肾上腺髓质BACE1蛋白水平下降,TH蛋白水平升高(P<0.05)。结论BACE1定位于大鼠肾上腺髓质神经纤维;BACE1水平下调可能参与减缓CIH引起的交感神经活性过度增强。

Objective To observe the expression and localization ofβ-site amyloid precursor protein cleaving enzyme 1(BACE1)in rat adrenal gland and to detect the effect of cyclic intermittent hypoxia(CIH)on the expression of BACE1.Methods The expression and localization of BACE1 in rat adrenal gland were detected by Western blotting and immunohistochemistry.Sixteen male Sprague-Dawley(SD)rats were randomly divided into two groups:control group and CIH group,8 rats in each group.The protein levels of BACE1 and tyrosine hydroxylase(TH)in rat adrenal medulla were detected by Western blotting after CIH 2 weeks treatment.Results BACE1 was mainly localized in rat adrenal medullary nerve fibers.Compared with the control group,BACE1 protein level decreased and TH protein level increased in the adrenal medulla in the CIH group.Conclusion BACE1 is located in rat adrenal medullary nerve fibers.The decreased level of BACE1 may participate in slowing down the excessive enhancement of sympathetic activity induced by CIH.