芦丁对丙烯酰胺染毒大鼠胼胝体髓鞘蛋白表达的影响

Protective effect of Rutin on myelin toxicity in the corpus callosum of rats induced by acrylamide

目的探讨芦丁(Rut)对丙烯酰胺(ACR)染毒大鼠胼胝体髓鞘碱性蛋白(MBP)及髓鞘蛋白脂蛋白(PLP)表达的影响。方法成年SD雄性大鼠32只,随机分为4组:对照组、20 mg/kg丙烯酰胺染毒组(ACR)、100mg/kg Rut保护组(R1+ACR)、200 mg/kg Rut保护组(R2+ACR),每组8只,灌胃21 d后取材。每周1次步态评分,观察大鼠的步态变化;免疫组织化学及Western blotting检测各组大鼠MBP及PLP表达量的变化。结果步态评分结果显示,与对照组相比,ACR组步态评分随染毒时间延长而增加,R1+ACR及R2+ACR组与对照组相比步态评分亦呈增加趋势,但与ACR组相比,步态评分明显降低(P<0.05);免疫组织化学及Western blotting结果显示,与对照组相比,ACR组胼胝体MBP及PLP的表达量均明显降低(P<0.01),与ACR组相比,R1+ACR及R2+ACR组MBP及PLP的蛋白表达量增加(P<0.05)。结论芦丁对丙烯酰胺染毒大鼠的髓鞘具有保护作用,这种保护作用可能与Rut抑制ACR染毒引起的胼胝体髓鞘蛋白MBP及PLP的减少有关。

Objective To study the effect of rutin(Rut)on the expression of myelin basic protein(MBP)and myelin protein lipoprotein(PLP)in corpus callosum of rats infected with acrylamide(ACR).Methods Thirty-two SD adult male rats were randomly divided into 4 groups:control,20 mg/kg acrylamide poisoning group(ACR),100 mg/kg Rut protection group(R1+ACR),200 mg/kg Rut protection group(R2+ACR),8 in each group,and were given gastric gavage for 21 days.The changes of the rats’gait were recorded weekly;Immunohistochemistry and Western blotting were used to detect the changes in the expression levels of MBP and PLP in each group of rats.Results The gait score results showed that the gait score of the ACR group increased with the extension of exposure time compared with the control group.The gait score of the R1+ACR group and R2+ACR group also showed an increase trend compared with the control group,but the gait score was significantly lower than that of the ACR group(P<0.05).Immunohistochemistry and Western blotting results showed that the expression of MBP and PLP in the corpus callosum of the ACR group was significantly decreased compared with the control group(P<0.01),while the expression of MBP and PLP in the R1+ACR group and R2+ACR group increased(P<0.05).Conclusion Rutin has a protective effect on myelin sheath in rats infected with acrylamide,which may be related to the inhibition of MBP and PLP in corpus callosum induced by ACR infection.