姜黄素对糖尿病大鼠氧化应激和脂代谢的影响及肾脏保护机制

Effects of curcumin on oxidative stress and lipid metabolism for kidney protection mechanism in diabetic rats

目的探讨姜黄素对糖尿病大鼠的氧化应激和脂代谢影响及保护肾脏的机制。方法将60只Wistar大鼠随机分为正常对照组(n=20)和糖尿病模型制备组(n=40)。正常对照组大鼠给予普通饲料喂养,糖尿病模型制备组大鼠以高糖高脂饲料喂养。饲养4周后,糖尿病模型制备组大鼠一次性腹腔注射链脲佐菌素60 mg/kg,造模成功后35只随机分为糖尿病模型组(n=17)、姜黄素组(n=18)。糖尿病模型组继续喂以高糖高脂饮食,姜黄素组每天给予高糖高脂饲料和200 mg/(kg·d)姜黄素(1%羧甲基纤维钠制备成混悬液)干预,同时正常对照组和糖尿病模型组灌服同体积1%羧甲基纤维钠。8周后检测各组大鼠体重、肾脏指数、血糖、24 h尿微量白蛋白排泄率(UAER)、血肌酐、尿素氮、血脂、血清和肾皮质超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)活性和丙二醛(MDA)含量。结果与正常对照组比较,糖尿病模型组大鼠的体重下降,肾脏指数、血糖、血肌酐、尿素氮、甘油三酯、胆固醇和24 h UAER含量显著升高,血清及肾皮质中MDA含量升高(P<0.05),血清及肾皮质SOD、CAT和GSH-Px活力显著降低(P<0.05);与糖尿病模型组比较,姜黄素组体重增加,肾脏指数、血糖、血肌酐、尿素氮、甘油三酯、胆固醇和24 h UAER含量显著降低(P<0.05),血清及肾皮质中MDA含量降低,同时血清及肾皮质SOD、CAT和GSH-Px活力显著升高(P<0.05)。结论在糖尿病大鼠中,姜黄素可能通过抑制血清及肾脏的氧化应激和降低血脂水平发挥肾脏的保护作用。

Objective To investigate the effects and the mechanism of renal protection of curcumin on oxidative stress and lipid metabolism in diabetic rats.Methods 60 Wistar rats were randomly divided into normal control group(n=20)and diabetic model preparation group(n=40).Rats in the normal control group were fed with normal diet,while rats in the diabetes model group were fed with high sugar and high fat diet.After 4 weeks of feeding,rats in the diabetic model preparation group were injected with streptozotocin(60 mg/kg)intraperitoneally once.A total of 35 rats were successfully modeled and then randomly divided into diabetic model group(n=17)and curcumin group(n=18).The diabetic model group continued to be fed with high sugar and high fat diet,and the curcumin group was given high sugar and high fat diet and 200 mg/(kg·d)curcumin(1%carboxymethyl fiber sodium prepared into suspension)intervention every day;while the normal control group and the diabetic model group were given 1%carboxymethyl fiber sodium of the same volume.The body weight,kidney index,blood glucose,serum creatinine,urea nitrogen,blood lipid and 24-hour urinary microalbumin excretion rate(24 h UAER)of rats in each group were measured.Superoxide dismutase(SOD),catalase(CAT),glutathione peroxidase(GSH-Px)and m-alondialdehyde(MDA)in serum and kidney tissues were measured.Results Compared with the control group,the levels of the body weight,kidney index,blood glucose,serum creatinine,urea nitrogen,triglyceride,total cholesterol and 24 h UAER in the diabetic model group increased significantly.The level of MDA in serum and kidney tissues increased significantly(P<0.05),while the levels of SOD,CAT and GSH-Px activities in serum and kidney tissues decreased significantly(P<0.05).Compared with diabetic model group,the levels of the body weight increased,while the kidney index,blood glucose,serum creatinine,urea nitrogen,triglyceride,total cholesterol and 24 h UAER in the curcumin group decreased significantly,the level of MDA in serum and kidney tissues decreased significantly(P<0.05);the levels of SOD,CAT and GSH-Px in serum and kidney tissues increased significantly(P<0.05)in the curcumin group.Conclusions Curcumin may play a renal protective role in diabetic rats by inhibiting oxidative stress in serum and kidney and reducing blood lipid.