miRNA-25对中国不同恶性肿瘤患者诊断价值的Meta分析

Diagnostic value of miRNA-25 for different cancers in Chinese population:a Meta-analysis

目的通过Meta分析评价中国人群中miRNA-25在不同恶性肿瘤中的诊断价值。方法检索中国知网、维普、万方、Pubmed、Embase中关于miRNA-25对恶性肿瘤诊断价值的研究,发表时间从建库至2019年10月,提取数据后采用Meta.DiSc1.4软件进行初步统计分析,Review Manager 5.3的QUADAS-2工具对纳入文献进行质量控制,STATA 15.0软件分析是否存在发表偏倚。结果共10篇纳入研究,其中病例组792例,对照组715例。合并灵敏度(SEN)为0.71(95%CI:0.69~0.74),合并特异度(SPE)为0.84(95%CI:0.81~0.86),合并阳性似然比(PLR)为4.29(95%CI:3.39~5.42),合并阴性似然比(NLR)为0.35(95%CI:0.31~0.40)。合并诊断优势比(DOR)为13.09(95%CI:9.64~17.78),SROC(Summary ROC)曲线显示曲线下面积(AUC)为0.80(95%CI:0.77~0.84)。亚组分析显示,miRNA-25对于不同恶性肿瘤的诊断价值提示肺癌组的诊断价值及特异度最高(DOR=16.418、SPE=0.863)。STATA 15.0分析研究未发现显著性发表偏倚(P=0.21)。结论中国人群中外泌体miRNA-25对不同恶性肿瘤,尤其是肺癌,有较高的灵敏度和特异度,可作为一项潜在的筛查生物标记物用于辅助诊断。

Objective Meta analysis was used to evaluate the diagnostic value of miRNA-25 in different malignant tumors in Chinese population.Methods The research on the diagnostic value of miRNA-25 in China national knowledge internet(CKNI),VIP,Wanfang,PubMed and EMBase was searched.The publication time was from the establishment of database to October 2019.After the data was extracted,the Meta.DiSc1.4 software was used for preliminary statistical analysis.The Review Manager 5.3's QUADAS-2 tool was used for quality control of the incoming literature.The STATA 15.0 software was used to analyze whether there was publication bias.Results A total of 10 articles were included in the study,including 792 cases in the case group and 715 cases in the control group.The combined sensitivity,combined specificity,combined specificity,combined positive likehood ratio(PLR),negative likehood ratio(NLR)were 0.71(95%CI:0.69-0.74),0.84(95%CI:0.81-0.86),4.29(95%CI:3.39-5.42),0.35(95%CI:0.31-0.40),respectively.Diagnostic odds rate(DOR)was 13.09(95%CI:9.64-17.78),and the area under curve(AUC)was 0.80(95%CI:0.77-0.84).Subgroup analysis showed that the diagnostic value of miRNA-25 for different malignant tumors suggested that the lung cancer group had the highest diagnostic value and specificity(DOR=16.418,SPE=0.863).Funnel plot indicated that no significant publication bias was found(P=0.21).Conclusions miRNA-25 is highly sensitive and specific to different malignancies,especially lung cancer,and can be used as a potential screening biomarker to assist diagnosis.