野生和人工饲喂条件下甘肃鼢鼠肠道细菌多样性比较

Comparison of intestinal bacterial diversity of Gansu zokor under wild and artificial feeding conditions

【目的】肠道微生物在宿主代谢和健康方面发挥着重要的作用,已有报道表明甘肃鼢鼠肠道菌群和地域、性别以及季节等因素有关,但对于野生和人工饲喂条件下,取食不同食物的甘肃鼢鼠肠道菌群是否存在差异尚不明确。【方法】本研究选取苜蓿、樟子松根、油松根和云杉根对甘肃鼢鼠进行分组饲喂,同时以野生个体作为对照,采用16S rRNA V3-V4高通量测序技术对野生和人工饲喂甘肃鼢鼠肠道细菌进行比较。【结果】野生和人工饲喂条件下,优势菌门均为Firmicutes和Bacteroidetes,但含量却存在显著性差异。在人工饲喂条件下,樟子松根、油松根和云杉根饲喂的三组甘肃鼢鼠肠道细菌群落结构相似度较高,而与苜蓿组和野生组之间均存在较大差异,樟子松组、油松组和云杉组甘肃鼢鼠肠道细菌多样性高于苜蓿组和野生组。【结论】野生和人工饲喂条件下甘肃鼢鼠肠道菌群组成和结构存在显著性差异。

[Objective]Intestinal bacteria play an important role in host metabolism and health.It has been reported that the intestinal flora of Gansu zokor is related to regional,gender and seasonal factors,however,it is not clear whether there is a difference in the intestinal bacterial of Gansu zokor feeding with different feeds under wild and artificial feeding conditions.[Methods]In this study,we selected alfalfa,the roots of Mongolian,Chinese pine and spruce for feeding groups,wild Gansu zokors captured in the same habitat as controls.Bacterial diversity of digestive tract contents in Gansu zokor was analyzed by 16S rRNA V3-V4 high-throughput sequencing technology.[Results]Under the conditions of wild and artificial feeding,the dominant bacteria were both Firmicutes and Bacteroidetes,but the content was significantly different.Under artificial feeding conditions,the intestinal bacterial community structure of Mongolian pine,Chinese pine and spruce was similar,they all had significant difference with alfalfa and wild groups.The intestinal bacterial diversity of Mongolian pine,Chinese pine,spruce were higher than alfalfa and wild groups.[Conclusion]The results showed that there were significant differences in the composition and structure of intestinal bacterial of Gansu zokor under wild and artificial feeding conditions.