摘要
Isochorismate synthase(ICS) plays an essential role in the accumulation of salicylic acid(SA) and plant disease resistance. Diseases caused by Botryosphaeria dothidea affect apple yields. Thus, it is important to understand the role of ICS1 in disease resistance to B. dothidea in apple. In this study, SA treatment enhanced the resistance to B. dothidea. Md ICS1 was induced by B. dothidea and enhanced the resistance to B. dothidea. Md ICS1 promoter analysis indicated that the W-box was vital for the response to B. dothidea treatment. Md WRKY15 was found to interact with the W-box using yeast one-hybrid screening. Subsequently, the interaction was confirmed by EMSA, yeast one-hybrid, Ch IP-PCR, and quantitative PCR assays. Moreover, luciferase and GUS analysis further indicated thatMd ICS1 was transcriptionally activated by Md WRKY15. Finally, we found the function of Md WRKY15 in the resistance to B. dothidea was partially dependent on Md ICS1 from the phenotype of transgenic apples and calli. In summary, we revealed that Md WRKY15 activated the transcription of Md ICS1 by directly binding to its promoter to increase the accumulation of SA and the expression of disease-related genes, thereby resulting in the enhanced resistance to B.dothidea in the SA biosynthesis pathway.
基金
supported by grants from the National Key Research and Development Program(2018YFD1000200)
the National Natural Science Foundation of China(31772275,U1706202)
the Natural Science Fund for Excellent Young Scholars of Shandong Province(ZR2018JL014)。
