摘要
目的探讨血管生成素1(Ang-1)在瘢痕成熟过程中的表达。方法取雌雄不限的新西兰大白兔,体重均>2.0 kg,在每只兔子双侧耳腹侧中部相同位置制造瘢痕模型,观察瘢痕的大体形态,并分别在创面上皮化后1、2、4、8、12周切取兔耳瘢痕组织标本。在上皮化12周瘢痕组织标本收集后,于兔耳腹侧正常皮肤处收集正常皮肤组织,分别液氮保存行HE染色及Western印迹观察兔瘢痕的组织学变化及Ang-1在瘢痕成熟过程中的表达。结果瘢痕成熟过程中,瘢痕Ang-1表达趋势是先升高,自上皮化后1周0.22±0.04至上皮化后2周最高0.29±0.11;之后逐渐降低,上皮化后4周0.12±0.06,上皮化后8周0.05±0.01,至上皮化后12周最小0.00±0.00,接近正常皮肤Ang-1表达0.05±0.01,差异有统计学意义(P<0.05)。HE染色观察到瘢痕的变化表现为瘢痕的表皮层及真皮层逐渐增厚,至上皮化后4周左右最厚,细胞排列密集,以后瘢痕逐渐变薄,细胞密度进一步降低。结论Ang-1在瘢痕中表达的变化趋势与瘢痕形态的变化趋势大致相似。Ang-1在瘢痕成熟过程中可能起重要作用。
Objective To explore the expression of angiopoietin-1 in scar during the scar strophying.Methods The rabbit ear scar model was established in the same position of each ear ventral center skin regardless of gender.The weight of each rabbit was greater than 2.0 kg weight.The scars were collected at weeks 1,2,4,8,and 12.After 12 weeks of scar tissue samples were collected,together with normal skin tissues in the rabbit ear ventral normal skin.The tissues were preseved in 10%formalin liquid and cryopreserved in liquid nitrogen,respectively.The general form of scar tissues and the expression of Ang-1 in scar were investigated by hematoxylin and eosin staining and Western-blot.Results The expression of Ang-1 increased gradually,and the highest at 2 week after epithelial change as(0.29±0.11),then decreased gradually,and the lowset at week 12 as(0.00±0.00),which was close to normal skin expression as(0.05±0.01)(P<0.05).Conclusions Ang-1 may play an important role during the scar atrophying.
作者
吴子涵
李高峰
Wu Zihan;Li Gaofeng(Department of Burn and Plastic Surgery,Xiangxi Autonomous Prefecture People's Hospital,the First Affiliated Hospital of Jishou University,416000;Hunan Provincial People's Hospital Affiliated to Hunan Normal University,Changsha,Hunan Province,410006)
出处
《中华医学美学美容杂志》
2020年第1期67-70,共4页
Chinese Journal of Medical Aesthetics and Cosmetology
基金
湖南省自然科学基金(联合基金)(13116033)。
