生物除磷工艺中高效聚磷菌的快速筛选和鉴定

Rapid Screening and Identification of Efficient Phosphorus Accumulating Organisms in Biological Phosphorus Removal Process

通过厌氧/好氧交替平板迭代法培养活性污泥,采用5-溴-4-氯-3-吲哚基-磷酸盐(BCIP)蓝白斑筛选法、PHB染色、异染颗粒染色进行高效聚磷菌的快速筛选,并基于16 S rDNA对各菌株进行高通量测序和鉴定。结果表明,4株菌可以分解BCIP产生蓝绿色菌落且厌氧PHB和好氧异染颗粒染色为蓝黑色,除磷率分别为1号菌85.51%,2号菌91.06%,3号菌95.51%,4号菌89.13%。高通量测序结果表明,1号菌为Exiguobacterium sp.ZWU0009(微小杆菌属),2号菌为Bacillus sp.C10(芽孢杆菌属),3号菌为Exiguobacteriumacetylicum strain SI17(乙酰杆菌属),4号菌为Exiguobacteriumacetylicum strain BGSLP4(乙酰杆菌属)。

The activated sludge was cultured by plate iteration method with alternating anaerobic and aerobic operation.Efficient phosphorus accumulating organisms were rapidly screened using 5-bromo-4-chloro-3-indolyl-phosphate(BCIP),dyeing of PHB and heterochromatic particles.Each strain was sequenced and identified by high-throughput sequencing based on 16S rDNA.The results showed that the four strains could decompose BCIP and produce blue-green.After staining,the PHB in the anaerobic stage and the heterodye particles in the aerobic stage were all blue black.The phosphorus removal rates of the four strains were 85.51%(No.1 strain),91.06%(No.2 strain),95.51%(No.3 strain),89.13%(No.4 strain)respectively.The results of high-throughput sequencing showed that the No.1 strain was Exiguobacterium sp.ZWU0009,the No.2 strainwas Bacillus sp.C10,the No.3 strainwasExiguobacteriumacetylicum strain SI17,and the No.4 strainwasExiguobacteriumacetylicum strain BGSLP4.