乳酸菌利用低聚果糖和低聚木糖的特性研究

Fructooligosaccharides and Xylooligosaccharides Utilization Properties of Lactic Acid Bacteria

以35株乳酸菌(LAB)为研究对象,通过体外实验研究乳酸菌利用低聚果糖(FOS)和低聚木糖(XOS)的偏好性、代时和产酸速率。通过溴甲酚紫平板法发现4株鼠李糖乳杆菌、3株嗜热链球菌和2株嗜酸乳杆菌均不能利用FOS和XOS,8株植物乳杆菌都能利用FOS和XOS,而短乳杆菌、干酪乳杆菌、发酵乳杆菌、格氏乳杆菌、罗伊氏乳杆菌和双歧杆菌能选择性利用FOS和XOS。此外,以FOS/XOS作为唯一碳源时,通过比较乳酸菌的代时和产酸速率,发现菌株的生长速率和产酸速率呈正相关,乳杆菌产酸速率普遍大于双歧杆菌。实验结果表明:乳酸菌对FOS和XOS的利用特性不同,与乳酸菌的种属相关。

The study investigated the preference,generation time and acid production rate of 35 lactic acid bacteria(LAB)using fructooligosaccharide(FOS)and xylooligosaccharide(XOS)in vitro.None of the strains of Lactobacillus rhamnosus,L.acidophilus and Streptococcus thermophilus were able to utilize FOS and XOS in bromocresol purple plate.Eight strains of L.plantarum could take advantage of FOS/XOS,while FOS/XOS could be selectively used by L.brevis,L.casei,L.fermentum,L.casei,L.reuteri and Bifidobacteria.The generation time and acid production rate of LAB were also detected when using FOS or XOS as the sole carbon source.The growth rate of the strains was positively correlated with the acid production rate.The acidification rate of Lactobacillus was generally higher than that of Bifidobacteria.These results demonstrated that the utilization of oligosaccharides by LAB had strain specificity and regularity.