脂肪酸钙对应激肉鸡氧化还原状态和免疫功能的影响

Effects of Fatty Acid Calcium on Redox State and Immune Function in Stressed Broiler Chickens

为探究脂肪酸钙对应激肉鸡氧化还原状态和免疫功能的影响,将360只一日龄黄羽肉鸡随机分为4组,对照组(Ⅰ组)和应激组(Ⅱ组、Ⅲ组、Ⅳ组),各组饲料中添加的油脂分别为豆油(Ⅰ组)、豆油(Ⅱ组)、饲料级猪油(Ⅲ组)、豆油脂肪酸钙(Ⅳ组),每组9个重复栏,实验周期为63 d,检测肉鸡血液、肝脏和肠道中氧化还原状态和免疫功能相关的指标。结果如下:(1)Ⅳ组肉鸡空肠、血浆HSP70含量显著低于Ⅱ组和Ⅲ组(P<0.05),血浆ACTH、CORT含量显著低于Ⅱ组和Ⅲ组(P<0.05)且接近Ⅰ组。(2)Ⅳ组肝脏CAT活力、肝脏和十二指肠总SOD活力显著高于Ⅲ组(P<0.05),血清、肝脏和十二指肠MDA含量显著低于Ⅲ组(P<0.05),肝脏MDA含量显著低于Ⅱ组且与Ⅰ组无显著性差异。(3)Ⅳ组血浆IgG含量、血浆IgA含量显著高于Ⅲ组(P<0.05),Ⅳ组血浆、肝脏IL-6含量显著低于Ⅲ组(P<0.05)。以上结果表明,脂肪酸钙能够改善应激肉鸡体内的氧化还原状态,缓解应激对肉鸡免疫功能的不利影响,增强抗应激能力。

The aim of the present study was to evaluate the effect of fatty acid calcium on redox status and immune function of stressed broilers.A total of 360 yellow-feather broilers with 1-day-age were randomly divided into 4 groups:control(group I)and stress groups(group II,group III,group IV).The oils added in each group were soybean oil(group I),soybean oil(group II),feed grade lard(group III),soybean oil fatty acid calcium(group IV),with 9 repetitions per group.After 63 days,the redox status and immune function related indicators in blood,liver and intestine were detected.The content of HSP70 in jejunum and plasma of group IV was significantly lower than that of group II and III(P<0.05).The levels of ACTH and CORT in plasma of group IV were significantly lower than those of group II and III(P<0.05)and close to those of group I.The liver CAT activity,liver and duodenal total SOD activities in group IV were significantly higher than those in group III(P<0.05),and the MDA content in serum,liver and duodenum was significantly lower than that in group III(P<0.05).Liver MDA content was significantly lower than group II and there was no significant difference from groupⅠ.Plasma IgG and IgA contents in group IV were significantly higher than those in group III(P<0.05).The plasma and liver IL-6 levels in group IV were significantly lower than those in group III(P<0.05).The results indicated that,the redox state of stressed broilers was improved,the adverse effects of stress on the immune function of broilers was relieved,and the anti-stress ability was enhanced by fatty acid calcium.