摘要
构建了一种快速检测单核细胞增生李斯特氏菌的适配体试纸条。以单核细胞增生李斯特氏菌核酸适配体为识别分子,纳米金为显色信号,基于双适配体夹心原理,制备了用于检测食品中单核细胞增生李斯特氏菌的适配体试纸条,并对制备条件如适配体浓度、胶体金溶液pH值、纳米金-适配体复合物和链霉亲和素-纳米金复合物的包被量等进行了优化。结果显示,在优化条件下,该试纸条对单核细胞增生李斯特氏菌的裸眼可视检测限为103 cfu/mL,且具有很高特异性。可实现样品中单核细胞增生李斯特氏菌的快速定性检测。
An aptamer-based lateral flow strip was developed for the rapid detection of Listeria monocytogenes.This assay was designed based on the sandwich-type format by using primary aptamer conjugated with gold nanoparticles(AuNP)as a signaling probe and secondary aptamer coated membrane as a capturing probe.Several experimental conditions were investigated,including the concentration of aptamer,pH of AuNP solution,and loading quantity of AuNP-aptamer conjugates and streptavidin-aptamer conjugates.Under optimized conditions,the aptamer-based lateral flow strip showed a visual limit of detection(LOD)of 103 cfu/mL,and had no significant cross-reactivity with several food-borne pathogens.The aptamer-based lateral flow strip was shown to provide a reliable,accurate method for rapid detection of Listeria monocytogenes in the real samples.
作者
卢春霞
李红敏
孙凤霞
康立超
陈亚
任江涛
马朝阳
LU Chunxia;LI Hongmin;SUN Fengxia;KANG Lichao;CHEN Ya;REN Jiangtao;MA Zhaoyang(Life Science and Technology Institute,Yangtze Normal University,Chongqing 408100,China;Analysis and Testing Center,Xinjiang Academy of Agriculture and Reclamation Science,Shihezi 832000,China;Chongqing Fuling Institute for Food and Drug Control,Chongqing 408100,China;School of Food Science and Technology,Jiangnan University,Wuxi 214122,China)
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2020年第4期85-92,共8页
Journal of Food Science and Biotechnology
基金
重庆市涪陵区科技计划项目(FLKW,2017ABB1038)。
