microRNA-138通过靶向作用于CCND1抑制结肠癌细胞增殖

microRNA-138 inhibits colon cancer cell proliferation by targeting CCND1

目的探讨人结肠癌细胞SW620中细胞周期蛋白1(CCND1)的表达情况及其异常表达对细胞增殖的影响,阐述microRNA-138(miR-138)在SW620增殖中的可能机制。方法采用实时荧光定量聚合酶链式反应(qRT-PCR)以及Western blot确定结肠癌组织和癌旁组织中CCND1的表达情况;随后采用qT-PCR检测结肠癌组织内miR-138的表达,并通过统计学分析miR-138与CCND1表达的相关性,培养SW620细胞并转染miR-138模拟物(mimics)后验证miR-138与CCND1的相关性,在SW620细胞内进一步转染miR-138模拟物后,Transwell和MTT检测不同处理组中细胞增殖和迁移能力的改变情况;继续转染CCND1 siRNA后,探索miR-138影响细胞生物学过程的可能机制。结果结肠癌组织中,与相应癌旁组织相比,CCND1表达显著升高,miR-138表达降低,差异有统计学意义(P<0.05);转染miR-138mimics后,与空白对照组(未转染组)相比,细胞迁徙能力下降,同时增殖降低,差异有统计学意义(P<0.05);转染CCND1 siRNA后,与未转染组相比,miR-138表达变化无显著差异(P>0.05),而细胞侵袭能力和增殖能力较未转染组相比显著改变,差异有统计学意义(P<0.05)。结论结肠癌组织中CCND1异常升高可能与miR-138的表达降低有关,且miR-138影响SW620细胞的迁移和增殖能力可能是通过影响CCND1的表达实现的。

Objective To investigate the expression of CCND1 in human colon cancer cell line SW620 and its effect on cell proliferation,and to explain the possible mechanism of miR-138 in SW620 proliferation.Methods The expression of CCND1 in colon cancer tissues and paracancerous tissues was determined by qRT-PCR and Western blot.The expression of miR-138 in colon cancer tissues was detected by qT-PCR,and the correlation of expression of miR-138 and CCND1 was statistically analyzed.After the SW620 cells were cultured and transfected with miR-138 mimics,the correlation between miR-138 and CCND1 was verified.After transfection of miR-138 mimics in SW620 cells,Transwell and MTT were used to detect changes in cell proliferation and migration capacity in different treatment groups,as well as the possible mechanism by which miR-138 affected cell biology processes after continued transfection of CCND1 siRNA.Results In colon cancer tissues,CCND1 expression was significantly increased and miR-138 was abnormally decreased compared with the corresponding adjacent tissues.The difference was statistically significant(P<0.05).After transfection of miR-138mimics,the control group was not compared with the transfection group,the cell migration ability decreased and the proliferation decreased,and the difference was statistically significant(P<0.05).After transfection of CCND1 siRNA,the expression of miR-138 was not significantly changed compared with the untransfected group.The invasive ability and proliferative capacity of the cells were significantly higher than those of the untransfected group,and the difference was statistically significant(P<0.05).Conclusion The abnormal increase of CCND1 in colon cancer may be related to the decreased expression of miR-138,and the ability of miR-138 to affect the migration and proliferation of SW620 cells may be affected by the expression of CCND1.