虫草素调节非小细胞肺癌细胞株细胞周期、凋亡及相关抑癌基因的实验研究

Experimental study of cordycepin regulating cell cycle,apoptosis and related tumor suppressor genes in non-small cell lung cancer cell lines

目的 研究虫草素对非小细胞肺癌细胞株H358细胞周期、凋亡及相关抑癌基因的调节作用。方法 培养非小细胞肺癌细胞株H358并分组,空白对照组用不含药物的DMEM处理,虫草素组用含有50μmol/L、100μmol/L、200μmol/L虫草素的DMEM处理,处理24 h后测定细胞周期分布、细胞凋亡率、抑癌基因表达水平。结果 不同剂量虫草素处理后,H358细胞的S期比率、凋亡率及PTEN、p53、p16及Caspase-3、Caspase-8、Caspase-9的mRNA表达水平明显高于空白对照组,G2/M期比率明显低于对照组(P <0. 05),且200μmol/L虫草素处理后,H358细胞的S期比率、凋亡率及PTEN、p53、p16及Caspase-3、Caspase-8、Caspase-9的mRNA表达水平明显高于其他浓度虫草素处理的细胞,G2/M期比率明显低于其他浓度虫草素处理的细胞(P <0. 05)。结论 虫草素处理非小细胞肺癌细胞株能够使细胞周期停滞、细胞凋亡加剧,增加抑癌基因表达是虫草素发挥以上调节作用的分子途径,并呈剂量依赖性。

Objective To study the regulatory effect of cordycepin on cell cycle,apoptosis and related oncogene of non-small cell lung cancer cell line H358.Methods H358 cells were cultured and grouped,blank control group was treated with DMEM without drug,cordycepin group was treated with DMEM containing 0μmol/L,100μmol/L,200μmol/L cordycepin.Cell cycle distribution,apoptosis rate and expression of tumor suppressor genes were measured after 24 hours of treatment.Results After different doses of cordycepin treatment,the S-phase ratio,apoptotic rate and the expression levels of PTEN,p53,p16,Caspase-3,Caspase-8 and Caspase-9 in H358 cells were significantly higher than those in the blank control group.The G2/M phase ratio was significantly lower than that in the blank control group(P<0.05).After 200μmol/L cordycepin treatment,the S-phase ratio,apoptotic rate and the expression levels of PTEN,p53,p16,Caspase-3,Caspase-8 and Caspase-9 in H358 cells were significantly higher than those of other cordycepin-treated cells,and the G2/M phase ratio was significantly lower than that of other cordycepin-treated cells(P<0.05).Conslusion Cordycepin can arrest cell cycle and aggravate cell apoptosis in non-small cell lung cancer cell lines,increasing the expression of tumor suppressor genes is a molecular pathway for cordycepin to play the above regulatory role.