体外培养鼠胚实验对IVF实验室质控的评估

In vitro Culture Mouse Embryo Assay for Evaluating the Quality Control of IVF Laboratory

目的:新建IVF(体外受精)实验室在正式运行前需要按时序对实验室环境、培养箱、人员操作实施质量控制。质量控制的结果要通过体外培养鼠胚实验进行评估,合格后方可使用。方法:检测热通风处理后实验室VOC(挥发性有机化合物);利用血气分析仪检测培养液来设置培养箱;利用人精子体外存活实验验证;手术获得昆明种小鼠雌雄配子,行体外受精培养观察鼠胚的受精率、卵裂率、囊胚率;IVF实验室行业内标准是培养2细胞胚胎发育到囊胚的比率≥80%证明实验合格。结果:检测实验室环境值均符合标准;培养箱设定CO2浓度7%可正常使用;体外培养鼠胚实验受精率80.9%、卵裂率97.6%、囊胚率87.1%。结论:实验室质控方法行之有效,IVF实验室可投入使用。

Objective:Before the new IVF laboratory is put into operation,quality control of laboratory environment,incubator and personnel operation should be carried out in time.The results of quality control should be evaluated by in vitro culture of mouse embryos,which can be used only after qualified.Methods:Detecting the VOC after heat ventilation treatment;using the blood gas analyzer to detect the culture solution to set the incubator;verifying the survival test of human sperm in vitro;obtaining the male and female gametes of Kunming mice,and in vitro fertilization culture to observe the fertilization rate and cleavage of the mouse embryo rate,blastocyst rate;if the ratio of 2-cell embryo development to blastocyst is≥80%,the test is qualified.Results:The environmental values of testing laboratories are in line with the standards;the incubator with 7%CO2 concentration can be used normally;the in vitro fertilization rate was 80.9%,the cleavage rate was 97.6%,and the blastocyst rate was 87.1%.Conclusion:Laboratory quality control method is effective,and IVF laboratory can be put into use.