斜阳岛珊瑚礁区海泥可培养细菌的分离鉴定及其ECP抑菌作用

The Isolation,Identification and Its ECP Antibacterial Effect of Culturable Bacteria from Sea Mud in Coral Reef Area of Xieyang Island

为探究斜阳岛珊瑚礁区海洋微生物的代谢产物活性,为天然材料制备新药物资源的开发提供一定的理论依据和实践指导,本研究从斜阳岛珊瑚礁区海泥分离纯化得到3株菌株,经鉴定分别为蜡状芽孢杆菌(Bacillus cereus)、Sphingopyxis terrae和Fictibacillus phosphorivorans,分别培养并提取3株细菌的胞外产物(extracellular products,ECP),与硝酸银制备成生物纳米银,OD法测定其抑菌(溶藻弧菌)动力曲线,用2倍稀释法测出3株菌的最小抑菌浓度(MIC)和最小杀菌浓度(MBC)分别是21.2μg/mL、18.8μg/mL、20.0μg/mL,用牛津杯法分别测定了3株菌与溶藻弧菌的抑菌活性,抑菌直径分别是(12.20±0.18)mm、(1.32±0.12)mm、(10.81±0.15)mm,安全性实验表明此3株菌对鱼体是安全的。本实验对斜阳岛珊瑚礁区海泥中可培养细菌进行了初步分离与鉴定,并利用分离菌的胞外产物合成生物纳米银,结果显示其具有一定的抑菌效果,从而证明其具有开发新的天然防腐剂和新药的潜力。

In order to explore the activity of metabolites of marine microorganisms in coral reef area of Xieyang Island,and to provide certain theoretical basis and practical guidance for the development of new drug resources prepared from natural materials,in this experiment,three strains were isolated from the coral reef of Xieyang Island,and they were identified by biochemical tests as Bacillus cereus,Sphingopyxis terrae and Fictibacillus phosphorivorans.Extracellular products(ECP)of three strains were extracted respectively,and prepared into biological silver nanoparticles.The OD measurement method was applied to evaluate the bacteriostatic power curve.The minimum inhibitory concentration(MIC)and the minimum bactericidal concentration(MBC)were measured with a two-fold dilution method,and they were 21.2μg/mL,18.8μg/mL,20.0μg/mL.Oxford cup method was used to test the bacteriostatic activity against the Vibrio alginolyticus,and the antibacterial diameter were(12.20±0.18)mm,(11.32±0.12)mm,(10.81±0.15)mm.Safety test showed that three strains were safe for Tilapia mossambica.In this experiment,culturable bacteria in the sea mud of the coral reef area of Xieyang Island were preliminarily isolated and identified.The biological nano silver was synthesized from the extracellular products of the isolated bacteria.The results showed that it had certain antibacterial effect,thus proving its potential to develop new natural preservatives and new drugs.