白灵菇多糖对运动小鼠氧化应激的影响

Effect of Plesurotus nebrodensis Polysaccharide on Oxidative Stress in Exercised Mice

体外研究表明,白灵菇多糖具有较高的清除自由基能力。然而,白灵菇多糖在体内对运动引起的氧化应激的影响尚不清楚。本研究将100只SPF级雄性昆明小鼠随机分为5组,低剂量组、中剂量组和高剂量组昆明小鼠分别按照50 mg·kg^-1·d^-1、100 mg·kg^-1·d^-1和200 mg·kg^-1·d^-1的剂量灌胃白灵菇多糖溶液,对照组和模型组昆明小鼠灌胃等体积的蒸馏水,连续灌胃4周。研究显示,白灵菇多糖溶液以浓度依赖性方式提高了小鼠的力竭游泳时间(p<0.05)。白灵菇多糖以浓度依赖性方式降低了小鼠血清AST、ALT和CK水平,并显著减少了骨骼肌的病理变化(p<0.05)。白灵菇多糖以剂量依赖方式升高力竭游泳小鼠体内SOD、CAT和GSH-PX水平,并降低了MDA水平(p<0.05)。此外,对小鼠灌胃白灵菇多糖可以剂量依赖方式提高小鼠血清总抗氧化活性(p<0.05)。上述研究表明,白灵菇多糖可有效提高力竭游泳小鼠的抗疲劳能力,减轻运动引起的心肌、肝脏、骨骼肌和氧化应激损伤,提高机体的抗氧化能力。

In vitro studies have shown that Pleurotus nebrodensis polysaccharide has a high ability to scavenge free radicals.However,the effect of Pleurotus nebrodensis polysaccharides on exercise-induced oxidative stress in vivo is unclear.In this study,100 SPF male Kunming mice were randomly divided into 5 groups.The low-dose,middledose and high-dose Kunming mice were given Pleurotus nebrodensis polysaccharides solution at 50 mg·kg^-1·d^-1,100 mg·kg^-1·d^-1 and 200 mg·kg^-1·d^-1 respectively.The control group and the model group were intragastrically administrated with the same volume of distilled water for 4 weeks.Studies showed that Pleurotus nebrodensis polysaccharide solution increased the exhaustive swimming time of mice in a concentration-dependent manner(p<0.05).Pleurotus nebrodensis polysaccharide reduced serum AST,ALT and CK levels in a concentration-dependent manner and significantly reduced pathological changes in skeletal muscle(p<0.05).Pleurotus nebrodensis polysaccharide increased the levels of SOD,CAT and GSH-PX in exhausted swimming mice in a dose-dependent manner and decreased MDA levels(p<0.05).In addition,the administration of Pleurotus nebrodensis polysaccharides in mice increased the total antioxidant activity of the mice in a dose-dependent manner(p<0.05).The above studies showed that Pleurotus nebrodensis polysaccharide could effectively improve the anti-fatigue ability of exhausted swimming mice,reduce the myocardial,liver,skeletal muscle and oxidative stress damage caused by exercise,and improve the body’s antioxidant capacity.