血清神经生长因子及细胞免疫水平在乳腺癌中的表达及其与临床病理特征的相关性

Expression of serum nerve growth factor and cellular immunity in breast cancer and its correlation with clinicopathological feature

目的探讨血清神经生长因子(NGF)及细胞免疫水平在乳腺癌患者中的表达及其与临床病理特征的相关性。方法选择2015年3月~2018年2月在杭州市妇产科医院及浙江省舟山医院确诊的乳腺癌患者49例,设为观察组;选择同期入院的乳腺良性疾病患者45例,设为对照组;选择同期入院健康体检女性47名,设为正常组。采用酶联免疫吸附试验测定各组NGF表达水平;采用流式细胞仪测定各组CD3^+、CD4^+、CD8^+及CD4^+/CD8^+水平;记录并统计患者临床病理资料,分析各组和观察组乳腺癌不同临床病理资料的NGF、细胞免疫水平;采用Pearson分析NGF、细胞免疫水平与乳腺癌患者临床病理资料的相关性。结果三组NGF、细胞免疫水平比较,差异均有统计学意义(P<0.05)。观察组NGF水平高于对照组与正常组,且对照组NGF水平高于正常组,差异均有统计学意义(P<0.05)。观察组CD3^+、CD4^+及CD4^+/CD8^+水平低于对照组与正常组,CD8^+水平高于对照组与正常组,差异均有统计学意义(P<0.05);对照组与正常组CD3^+、CD4^+、CD8^+及CD4^+/CD8^+水平比较,差异无统计学意义(P>0.05)。乳腺癌组织中NGF、CD3^+、CD4^+、CD8^+及CD4^+/CD8^+水平在年龄、病理组织学类型方面比较,差异无统计学意义(P>0.05);乳腺癌组织中NGF、CD3^+、CD4^+、CD8^+及CD4^+/CD8^+水平在肿瘤最大径、病理分级、分化程度及淋巴结转移方面比较,差异均有统计学意义(P<0.05);Pearson相关性分析显示:NGF、CD8^+水平与肿瘤最大径、病理分级、分化程度及淋巴结转移呈正相关(P<0.05);CD3^+、CD4^+及CD4^+/CD8^+水平与肿瘤最大径病理分级、分化程度及淋巴结转移呈负相关(P<0.05)。结论NGF在乳腺癌患者中呈高表达,导致机体细胞免疫水平下降,且二者表达水平与病理特征具有相关性,加强NGF及细胞免疫水平测定能评估患者疾病严重程度,指导临床治疗。

Objective To investigate the expression of serum nerve growth factor(NGF)and cellular immunity in breast cancer patients and its correlation with clinicopathological feature.Methods A total of 49 breast cancer patients diagnosed in Hangzhou Maternity Hospital and Zhejiang Zhoushan Hospital from March 2015 to February 2018 were selected as the observation group.A total of 45 patients with benign breast disease who were hospitalized at the same time were selected as the control group.A total of 47 female patients with physical examination at the same time were selected as the normal group.The expression levels of NGF in each group were determined by enzyme-linked immunosorbent assay.The levels of CD3^+,CD4^+,CD8^+ and CD4^+/CD8^+in each group were determined by flow cytometry.The clinicopathological data of the patients were recorded and counted.The levels of NGF and cellular immunity in each group and the levels of NGF and cellular immunity in different clinicopathological data of breast cancer in the observation group were analyzed.The correlation between NGF,cellular immunity level and clinicopathological data of breast cancer patients was analyzed by Pearson analysis.Results The levels of NGF and cellular immunity in the three groups were statistically significant(P<0.05).The level of NGF in the observation group was higher than that in the control group and the normal group,and the level of NGF in the control group was higher than that in the normal group,with statistically significant differences(P<0.05).The levels of CD3^+,CD4^+and CD4^+/CD8^+ in the observation group were lower than those in the control group and the normal group,and the level of CD8^+was higher than that in the control group and the normal group,with statistically significant differences(P<0.05).There was no significant difference in the levels of CD3^+,CD4^+,CD8^+ and CD4^+/CD8^+between the control group and the normal group(P>0.05).The levels of NGF,CD3^+,CD4^+,CD8^+ and CD4^+/CD8^+ in breast cancer tissues were not statistically significant differences in terms of age and histopathological type(P>0.05).The levels of NGF,CD3^+,CD4^+,CD8^+ and CD4^+/CD8^+in breast cancer tissues were compared in terms of tumor maximum diameter,pathological grading,differentiation degree and lymph node metastasis,with statistically significant differences(P<0.05).The Pearson correlation analysis results showed that the NGF and CD8^+ levels were positively correlated with the maximum tumor diameter,pathological grading,differentiation degree and lymph node metastasis(P<0.05).The levels of CD3^+,CD4^+ and CD4^+/CD8^+ were negatively correlated with the maximum tumor diameter,pathological grading,differentiation degree and lymph node metastasis(P<0.05).Conclusion The high expression of NGF in breast cancer patients leads to the decline of cellular immunity level in the body,and the expression level of both is correlated with the pathology feature.Strengthening the determination of NGF and cellular immunity level can assess the severity of the disease in patients and guide clinical treatment.