复方白虎汤对类风湿关节炎风寒湿痹证大鼠的治疗效果研究

Effects of Compound Baihu Decoction on Rats with Rheumatoid Arthritis of Wind-Cold-Damp Bi Syndrome

目的:探讨复方白虎汤对类风湿关节炎风寒湿痹证大鼠的治疗作用。方法:将SD大鼠随机分为空白对照组、风寒湿痹证组和复方白虎汤组,每组20只。制备类风湿关节炎风寒湿痹证大鼠模型,复方白虎汤组大鼠以复方白虎汤剂处理。观察大鼠的一般情况,记录发病时间及发病率,测量大鼠左后足跖厚度并采用关节炎指数(arthritis index,AI)量表评估关节炎程度,测定大鼠血清炎症因子[肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)及白细胞介素6(IL-6)]和免疫指标[类风湿因子(RF)、抗环瓜氨酸肽(Anti-CCP)抗体和C反应蛋白(CRP)]水平。结果:与风寒湿痹证组比较,复方白虎汤组大鼠的发病时间延长,发病率低,差异均有统计学意义(P<0.05)。从造模后9 d起,随着造模时间的延长,风寒湿痹证组大鼠足跖肿胀值和AI逐渐增加,造模后18 d达到最大值;造模后0、6、9、12、18、24及30 d,复方白虎汤组大鼠的足跖肿胀值明显低于风寒湿痹证组同期,差异均有统计学意义(P<0.05)。造模后9、12、18、24及30 d,复方白虎汤组大鼠AI指数明显低于风寒湿痹证组同期,差异均有统计学意义(P<0.05)。实验结束后,复方白虎汤组、空白对照组大鼠血清TNF-α、IL-1β、IL-6、RF、Anti-CCP抗体和CRP水平明显低于风寒湿痹证组,差异均有统计学意义(P<0.05)。结论:复方白虎汤可有效延长类风湿关节炎风寒湿痹证大鼠的发病时间,降低发病率,同时可能通过缓解炎症反应、调节免疫功能和改善关节肿胀症状来抑制踝关节病理改变。

OBJECTIVE:To probe into the treatment effects of compound Baihu Ddecoction on rats with rheumatoid arthritis of wind-cold-damp bi syndrome.METHODS:SD rats were randomly divided into the blank control group,the wind-cold-damp bi syndrome group and the compound Baihu decoction group,with 20 rats in each group.The rat model of rheumatic arthritis of wind-cold-damp bi syndrome was prepared,the rats from compound Baihu decoction group were treated with compound Baihu decoction.The general conditions of rats were observed,the onset time and incidence were recorded.The thickness of left hind paw of rats was measured,arthritis was evaluated by Arthritis index(AI)scale.The levels of serum inflammatory factors[tumor necrosis factorα(TNF-α),interleukin 1β(IL-1β)and interleukin 6(IL-6)]and immune indexes[rheumatoid factor(RF),anti-cyclic citrulline peptide(Anti-CCP)antibody,and C-reactive protein(CRP)]were detected.RESULTS:Compared with the wind-cold-damp bi syndrome group,the compound Baihu decoction group had longer onset time and lower incidence rate,and the differences were statistically significant(P<0.05).After modeling of 9 d,with the extension of modeling time,the swelling value and AI index of rats in the wind-cold-damp bi syndrome group increased,and reached the maximum value after modeling of 18 d.After modeling of 0,6,9,12,18,24 and 30 d,the swelling value of rats in the compound Baihu decoction group were significantly lower than those in the wind-cold-damp bi syndrome group,with statistically significant difference(P<0.05).After9,12,18,24 and 30 days,AI index of rats in compound Baihu decoction group was significantly lower than that in wind-cold-damp bi syndrome group,with statistically significant difference(P<0.05).After the experiment,the levels of TNF-α,IL-1β,IL-6,RF,Anti-CCP antibodies and CRP in the compound Baihu decoction group and the blank control group were significantly lower than those of the wind-cold-damp bi syndrome group,and the differences were statistically significant(P<0.05).CONCLUSIONS:Compound Baihu decoction can effectively prolong the onset time and reduce the incidence rate of rats with rheumatoid arthritis of wind-cold-damp bi syndrome.Meanwhile,the pathological changes of ankle joint may be inhibited by relieving the inflammatory response,regulating the immune function and improving the symptoms of joint swelling.