桑葚酒中花青素含量的测定方法优化

Optimization of Determination Method of Anthocyanin Content in Mulberry Wine

为确定pH示差法测定桑葚酒中花青素的最佳测定条件,本文对pH示差法测定桑葚酒中花青素含量的最佳测定波长、缓冲液的最适pH、平衡温度和时间等条件进行优化,并且对优化的pH示差法进行方法学评价。结果表明,在波长519 nm、缓冲液pH为1.0和4.5、于30℃下恒温40 min后测定桑葚酒中花青素含量是最佳的条件。测定桑葚酒中花青素的含量为23.80 mg/L。该方法的相对标准偏差(RSD)为2.076%,加标回收率为99.59%~102.70%,说明用优化后的pH示差法测定桑葚酒中花青素含量是准确可靠的。本文为酒为存在介质的花青素定量分析提供了借鉴。

In order to seek out the best condition to determine anthocyanin content in mulberry wine by pH differential spectroscopic method,the determination wavelength,pH value of buffer solution,equilibrium temperature and time were optimized.Then the optimized pH differential spectroscopic method was evaluated.The results showed that the best condition were when the wavelength was 519 nm,the pH of buffer solutions were 1.0 and 4.5,and kept at 30℃ for 40 min.The content of anthocyanin in mulberry wine was 23.80 mg/L.The relative standard deviation(RSD)of this method was 2.076%,and recoveries of the spiking samples were between 99.59% and 102.70%.The results demonstrate that it is accurate and reliable to determine the anthocyanin content in mulberry wine by the optimized pH differential spectroscopic method.This research provides some reference for the quantitative analysis of anthocyanin in alcoholic drink.