NaCl胁迫下柳树的转录组测序与初步分析

Transcriptome Sequencing and Preliminary Analysis of Willow under NaCl Stress

从组学水平分析盐胁迫下柳树内在分子机制,为柳树耐盐研究及耐盐基因的挖掘利用提供理论依据。本研究通过转录组测序技术对‘盐柳1号’(Salix psammophila’Yanliu No.1’)和‘渤海柳1号’(Salix matsudana’Bohailiu No.1’)经150 mmol/L NaCl胁迫处理后的叶片和正常叶片(对照)进行高通量转录组测序,并对获得的unigene进行从头组装和注释分析。结果表明:转录组测序共获得183987条Unigenes,平均长度为1080.18 bp,分别有120130条、140813条、98066条、88425条、47982条、83732条Unigenes被注释到NT、NR、COG、SwissProt、KEGG、COG和GO数据库,共有149864条(81.45%)Unigenes得到注释。在GO功能注释中,共得到55个GO功能小类,在KEGG代谢通路分析时,获得了135条KEGG通路。该转录组测序数据质量高,结果覆盖面广,为柳树耐盐基因挖掘和研究提供了一定的理论参考。

The objective of this study is to identify the inner molecular mechanism of willow under salt stress,which provided the theoretical basis for the research of salt tolerance and the exploitation of genes closely related to salt responsiveness.The transcriptome sequencing of the salt-tolerant the Salix psammophila’Yanliu No.1’and Salix matsudana’Bohailiu No.1’under normal blade(control)and 150 mmol/L NaCl stress were conducted and the dates were De novo assembled.The results showed that 183987 Unigenes were obtained by transcriptome sequencing,with an average length of 1080.18 bp.120130,140813,98066,88425,47982 and 83732 Unigenes were annotated into NT,NR,COG,SwissProt,KEGG,COG and GO databases respectively,and a total of 149864 Unigene(81.45%)were annotated.In the functional annotations of GO and KEGG,55 GO functional subclasses and 135 KEGG pathways were obtained respectively.The transcriptome sequencing data has high quality and wide coverage,and will provide a theoretical reference for the exploration and research of salt-tolerant genes in willows.