黄芩苷对糖尿病肾病大鼠肾纤维化的调控作用

Effect of baicalin on renal fibrosis in rats with diabetic nephropathy by TGF-β1

目的黄芩苷在降血糖血脂、改善代谢综合征方面的作用受到广泛关注,文章观察黄芩苷对糖尿病肾病(DN)大鼠肾的保护作用,探讨其可能的调控机制。方法45只雄性SD大鼠随机数字表法分为正常对照组、DN模型组、黄芩苷处理组,每组15只。正常组常规饲养,模型组与黄芩苷处理组建立DN模型,分别给予相应制剂灌胃治疗。固定时间点后检测大鼠体重、空腹血糖(FBG)、血清尿素氮(BUN)、血肌酐(Scr)水平,观察各组大鼠肾的病理组织学变化,Western blot检测转化生长因子-β1(TGF-β1)与α-SMA蛋白表达。结果与正常对照组大鼠相比,模型组大鼠空腹血糖在造模后4、8周明显升高,而体重降低(P<0.05);与模型组相比,黄芩苷治疗组大鼠在造模后4、8周血糖明显下降(P<0.05)。与正常对照组BUN、Scr、24 h尿量[(8.01±0.92)mmol/L、(24.15±3.24)μmol/L、(10.78±1.24)mL]相比,模型组[(16.97±3.63)mmol/L、(52.58±2.33)μmol/L、(32.65±2.69)mL]明显升高(P<0.05);而黄芩苷处理组较模型组[(12.73±0.67)mmol/L、(43.77±1.73)μmol/L、(19.34±2.16)mL]明显降低(P<0.05)。结论黄芩苷可延缓DN的进展,改善肾纤维化,其作用机制可能与黄芩苷通过低调节TGF-β1从而降低α-SMA的表达密切相关。

Objective Diabetic nephropathy(DN)is a pathological process involving the kidney that develops from Diabetes Mellitus(DM).The onset is hidden and difficult to reverse.Baicalin is an important flavonoid compound in Scutellaria Baicalensis.It has the functions of clearing heat and detoxifying,anti-inflammatory and anti-oxidant.In recent years,the role of baicalin in lowering blood glucose and lipids and improving metabolic syndrome has received widespread attention.Therefore,we observed the protective effect of baicalin on the kidneys of DN rats and explored its possible regulatory mechanism.Methods Forty-five male SD rats were randomly divided into a normal control group,a DN model group,and a baicalin-treated group,with 15 rats in each group.The normal group was routinely bred,and the DN model was established in the model group and the baicalin treatment group,and the corresponding preparations were given gavage treatment.After a fixed time point,rat body weight,fasting blood glucose(FBG),blood urea nitrogen(BUN),and serum creatinine(Scr)levels were measured,and the histopathological changes of the kidneys in each group were observed.TGF-β1 andα-SMA protein expression was measured by Western blot.Results Compared with the DN group,the FBG,BUN,Scr and 24h urine output of the baicalin treatment group were significantly decreased,and the body weight did not change significantly.Histopathological observation showed that compared with DN group,the pathological damage,glycogen deposition and fibrosis of the rats in the baicalin treatment group were significantly improved.Compared with the DN group,the results of Western blot showed that the expression of TGF-β1 andα-SMA protein in the baicalin treatment group were significantly decreased(P<0.05).Conclusion Baicalin can delay the progression of DN and improve renal fibrosis,and its mechanism may be closely related to the inhibition ofα-SMA expression by baicalin by low regulation of TGF-β1.