摘要
目的建立HPLC-MS/MS分析血液中美利曲辛和氟哌噻吨的方法。方法血液用甲醇/乙腈(1:9)提取后,用C18色谱柱进行分析,以水-甲醇(含0.1%甲酸)为流动相梯度洗脱,采用液相色谱-串联质谱SRM正离子模式检测。结果血中美利曲辛和氟哌噻吨的线性范围广,线性关系良好,相关系数r均大于0.999,美利曲辛和氟哌噻吨最低检测限分别为0.01ng/mL(S/N=8)、0.2ng/mL(S/N=12)。日内、日间精密度均小于10%。结论建立的方法准确度高、灵敏度好,可用于血液中美利曲辛和氟哌噻吨的同时定性、定量分析。
A HPLC-MS/MS method for the determination of melitracen and flupentixol in blood was established.After extracted with methanol/acetonitrile(1:9),they were analyzed by positive ion and SRM mode,ZORBAX Eclipse Plus C18 column.Water-methanol(containing 0.1%formic acid)was used as mobile phase gradient elution.The experimental results showed that melitracen and flupentixol in blood had a wide linear range and good linear relationship The correlation coefficient was greater than 0.999.The minimum detection limits of melitracen and flupentixol were 0.01ng/mL(S/N=8)and 0.2ng/mL(S/N=12),respectively.The intra-day and inter-day precision were both less than 10%.The method has high accuracy and good sensitivity.It can be used for simultaneous qualitative and quantitative detection of melitracen and flupentixol in blood.
作者
杨士云
李咏杰
乔静
张瑛
辛国斌
Yang Shiyun;Li Yongjie;Qiao Jing;Zhang Ying;Xin Guobin(Forensic Science Service of Beijing Public Security Bureau.Key Laboratory of Forensic Toxicology,Ministry of Public Security,Beijing,100192)
出处
《现代科学仪器》
2019年第6期18-21,共4页
Modern Scientific Instruments
