丹酚酸B对小鼠体内抗氧化和肠道微生物群落的影响

Effect of Salvianolic Acid B on Antioxidant Capacity and Intestinal Microflora in Mice

为研究丹酚酸B的体内抗氧化性和对小鼠肠道微生物群落的影响,本实验对昆明小鼠灌胃不同剂量丹酚酸B溶液,将小鼠分为丹酚酸B低剂量组(30 mg/kg mb)、丹酚酸B中剂量组(60 mg/kg mb)、丹酚酸B高剂量组(120 mg/kg mb)。42 d后,记录小鼠的体质量变化;计算心脏、肝脏、肾脏、脾脏的脏器指数;检测血液和肝脏的抗氧化指标。结果显示在小鼠肝脏中,中剂量、高剂量和阳性对照组相对于正常组脏器指数显著降低(P<0.05)。肾脏中,高剂量组相对于正常组脏器指数显著降低(P<0.05);阳性对照组相对于正常组极显著降低(P<0.01)。抗氧化指标检测结果显示,和正常组相比较,中剂量组血清中丙二醛(malondialdehyde,MDA)含量显著降低(P<0.05),高剂量组和VC组血清中MDA含量极显著降低(P<0.01),高剂量组肝脏中MDA含量显著降低(P<0.05)。高剂量组和VC组血清中GSH含量显著增加(P<0.05)。通过高通量测序技术分析可知,小鼠肠道菌群结构以拟杆菌门(Bacteroidetes)、厚壁菌门(Firmicutes)、变形菌门(Proteobacteria)、疣微菌门(Verrucomicrobia)、放线菌门(Actinobacteria)为各组的优势菌门。根据LEfSe分析发现相对正常组,3组给药组中Bacteroides vulgatus和Parabacteroides distasonis两个菌种相对丰度显著增加(P<0.05),相对丰度随药物浓度增加而降低。丹酚酸B可以提高小鼠体内抗氧化能力,改善小鼠肠道环境,增加有益菌,该发现为丹酚酸B治疗心血管疾病提供新的研究思路。

The purpose of this study was to investigate the in vivo antioxidant properties of salvianolic acid B and its effect on the intestinal microbial community in mice.Kunming mice were administered by gavage with salvianolic acid B solution at low(30 mg/kg mb),medium(60 mg/kg mb),and high(120 mg/kg mb)doses for 42 successive days.At 24 h after the last administration,body mass was recorded,the organ coefficients of heart,liver,kidney and spleen were calculated,and the antioxidant indexes of blood and liver were detected.The results showed that the organ coefficients of liver in the medium-dose,high-dose and VC control groups were significantly lower than that in the normal group(P<0.05).Similarly,the organ coefficient of kidney in the high-dose group was significantly lower than that in the normal(P<0.05)and VC control groups(P<0.01).The antioxidant indexes showed that compared with the normal group,MDA content was significantly decreased in the serum of mice in the middle-dose group(P<0.05)as well as the high-dose and VC control groups(P<0.01),and MDA content in the liver was also significantly decreased in the high-dose group(P<0.05).In contrast,serum glutathione(GSH)levels in the high-dose and VC groups were significantly increased(P<0.05).Through highthroughput sequencing analysis,the structure of intestinal flora in mice from each group was dominated by Bacteroides,Firmicutes,Proteobacteria,Verrucomicrobia and Actinobacteria.According to LEfSe analysis,the relative abundances of Bacteroides vulgatus and Parabacteroides distasonis were significantly increased in the three dose groups relative to the normal group(P<0.05),decreasing with increasing dose.Therefore,salvianolic acid B can improve the antioxidant capacity and the intestinal environment of mice,increasing the number of beneficial bacteria.This finding provides new ideas for the treatment of cardiovascular diseases with salvianolic acid B.