改良QuEChERS-高效液相色谱-串联质谱法检测植物油中的10种植物生长调节剂

Determination of ten plant growth regulators in vegetable oils by improved QuEChERS-high performance liquid chromatography-tandem mass spectrometry

采用改良QuEChERS技术作为前处理方法,建立了植物油中10种植物生长调节剂(PGRs)的高效液相色谱-串联质谱快速检测方法。样品用酸化乙腈提取,经增强型基质去除吸附剂(EMR-Lipid)净化,然后用高效液相色谱-串联质谱仪多反应监测模式检测,外标法定量。结果表明:方法线性范围1~100μg/L,10种PGRs线性关系良好,相关系数(r)为0.991~0.998;检出限为0.014~0.188μg/kg,定量限为0.048~0.625μg/kg;10种PGRs的回收率在70.2%~94.7%之间,RSD为1.2%~10.2%。该方法准确、快速、重复性好,适用于植物油中植物生长调节剂的测定。

A rapid method for the simultaneous determination of ten plant growth regulators in vegetable oils was developed by using high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS) based on modified QuEChERS sample pretreatment method.Samples were extracted with acidic acetonitrile,and cleaned up by Enhanced Matrix Removal Lipid(EMR-Lipid).The analytes were detected in the multi-reaction monitoring(MRM) mode and quantified by the external standard method.The calibration curves of ten plant growth regulators were linear well in the corresponding concentration ranges of 1-100 μg/L, with correlation coefficient(r) of 0.991-0.998. The limits of detection and quantification were 0.014-0.188 μg/kg and 0.048-0.625 μg/kg, respectively. The average recoveries of ten plant growth regulators ranged from 70.2% to 94.7% with relative standard deviations(RSDs) of 1.2%-10.2%.The method was quick,accurate,and sensitive, and was suitable for the determination of ten plant growth regulators in vegetable oils.