2型糖尿病患者与非糖尿病人群肠道菌群差异的研究

Differences of gut microbiota between type 2 diabetes and non-diabetic population

目的探究北京地区2型糖尿病与非糖尿病人群肠道菌群的差异。方法选取在北京协和医院就诊的2型糖尿病患者83例,同时选取64例非糖尿病人群作为对照组,收集研究对象及对照组的粪便样本,采用宏基因组测序技术进行肠道菌群的测定,比较2型糖尿病组与对照组的肠道微生物的差异。结果两组样本共检测出11个菌门,其中放线菌门(Actinobacteria)(P=0.013)、厚壁菌门(Firmicutes)(P=0.005)、梭杆菌门(Fusobacteria)(P=0.001)、变形菌门(Proteobacteria)(P<0.001)的物种多样性差异有统计学意义,其中放线菌门、梭杆菌门及变形菌门均在2型糖尿病患者中富集,厚壁菌门在对照组中富集。两组样本共检测出152个菌属,两组间有菌群丰度差异的菌属共有31个,在2型糖尿病组中,罗氏菌属(Roseburia)、真杆菌属(Eubacterium)、粪便杆菌属(Faecalibacterium)水平下降,双歧杆菌属(Bifidobacterium)、乳酸杆菌属(Lactobacillus)、大肠埃希菌(Escherichia)水平均增加。结论2型糖尿病组与对照组的肠道菌群物种构成存在显著差异,调节2型糖尿病患者的肠道菌群,可能有助于改善2型糖尿病患者病情。

Objective To explore the difference of gut microbiota between type 2 diabetes mellitus(T2DM)and non-diabetic population in Beijing.Methods 83 T2DM patients were selected as T2DM group and 64 non-diabetic subjects were selected as control group.Fecal samples were collected from all the subjects.The intestinal flora was detected by metagenome sequencing technology.Results 11 bacterialphyla were detected in the two groups,there were significant differences in species diversity of Actinobacteria(P=0.013),Firmicutes(P=0.005),Fusobacteria(P=0.001),Proteobacteria(P<0.001)between the two groups.Actinobacteria,Fusobacteria and Proteobacteria were all enriched in the T2DM group,Firmicutes were enriched in the control group.152 bacterial genera were detected in the two groups with 31 bacterial genera ofsignificant differences.In T2DM group,the levels of Roseburia,Eubacterium and Faecalibacterium decreased,while the levels of Bifidobacterium,Lactobacillus and Escherichia increased.Conclusion There are significant differ-ences in the composition of gut microbiota between T2DM patients and non-diabetic population.Regulation of gut microbiota in T2DM patients may be helpful to improve the condition of T2DM.