к-卡拉胶和黄原胶复合凝胶及其载药抗菌性能研究

A novel hydrogel based onк-carrageenan and xanthan with bacteriostatic performance

目的合成新型к-卡拉胶(к-CA)和黄原胶(XG)复合水凝胶,观测凝胶形貌、微结构和形成机制及其装载左氧氟沙星(LVFX)凝胶制剂的抗菌效果。方法利用溶胶-凝胶法合成к-CA/XG凝胶,使用扫描电子显微镜(SEM)、傅里叶红外光谱(IR)、X射线衍射(XRD)以及流变学等手段观测к-CA/XG凝胶结构、性质性能等;采用自制的牛津杯法对比测试LVFX凝胶制剂的抗菌效果。结果获得了新型к-CA和XG复合水凝胶体系以及LVFX凝胶新剂型。新型凝胶形貌表现为带状纹理为主的多形态微结构,在流变学上凝胶的应力屈服值只有25pa,表现为弱凝胶的性质;к-CA/XG凝胶的形成机制是天然多糖分子链之间依靠氢键作用通过螺旋形成纤维束带状纹理结构;试验表明,空白凝胶没有抑菌作用,LVFX凝胶制剂的杀菌效能可达到对照标准的50%。结论新型к-CA/XG复合凝胶合成,方法简便、成本低廉,同时利用该复合凝胶获得了新型抗菌药物凝胶制剂。

Objective To synthesize a novel hydrogel ofк-carrageenan(к-CA)and xanthan gum(XG)with mass ratio of 73 in presence of potassium ions,and investigate the morphology,microstructure,forming mechanism of the hydrogel and the antibacterial effect of the hydrogel loading with levofloxacin(LVFX).Methods The hydrogel was synthesized by sol-gel method,and its structure and properties were studied by means of scanning electron microscope,Fourier infrared spectroscopy,X-ray diffraction and rheology.The antibacterial effect of drug hydrogel was tested by Oxford cup method.Results A novel hydrogel system ofк-CA and XG,and its dosage forms with LVFX were obtained.The gel morphology showed mainly strip texture with polymorphic structure.In terms of rheology,the hydrogel only gave the stress yield value of 25 Pa as a weak gel.The gel forming mechanism was that the chains of two polysaccharides cross linked in the form of helix by hydrogen bonding and then the fibrous bundles formed owing to their aggregation.The bactericidal efficacy of the hydrogel with LVFX could reach about 50%to the positive antibacterial control.Conclusion A novel composite hydrogel can be synthesized with the natural polysaccharides by means of simple method and low cost suggesting new antibacterial gel preparation can also be developed.