响应面法优化酶法制备亚铁血红素肽工艺

Optimization of Enzymatic Preparation of Hemopeptides by Response Surface Methodology

在两步酶解的基础上,采用响应面法优化酶法制备亚铁血红素肽的工艺。以酶底比(E/S)、酶解时间、酶解温度为变量因素,以血红蛋白溶液水解度为响应值,进行Box-Behnken响应面试验设计并进行分析。结果表明:响应面法优化得到碱性蛋白酶的最佳酶解条件为E/S 10.16 kU/g、酶解时间5.45 h、酶解温度51.51℃;风味蛋白酶的最佳酶解条件为E/S 11.13 kU/g、酶解时间4.37 h、酶解温度48.34℃;通过验证实验证明,两步酶解后的实际水解度为54.31%。

The preparation of himopeptides by sequential hydrolysis with two enzymes was optimized using response surface methodology with Box-Behnken design. Enzyme-to-substrate ratio(E/S), hydrolysis time and temperature were taken as independent variables. The response variable was the degree of hydrolysis of hemoglobin. The results showed that the optimal hydrolysis conditions for alkaline protease were as follows: E/S 10.16 kU/g, time 5.45 h, temperature 51.51 ℃. The optimal hydrolysis conditions for flavourzyme were as follows: E/S 11.13 kU/g, time 4.37 h, temperature 48.34 ℃. The validation experiments showed that the degree of hydrolysis was 54.31% under these optimized conditions.