DADS通过Rac1/LIMK1/cofilin1通路增强沉默LIMK1抑制人胃癌BGC823细胞迁移侵袭

DADS enhanced LIMK1 silencing to inhibit migration and invasion of human gastric cancer BGC823 cells by Rac1/LIMK1/cofilin1 pathway

探讨二烯丙基二硫(DADS)通过Rac1/LIMK1/cofilin1通路对沉默LIMK1抑制BGC823细胞迁移侵袭的影响。划痕实验和侵袭实验观察迁移和侵袭能力;RT-PCR、Western blot、免疫组化检测LIMK1、Rac1、Rock1、Pak1与Cofilin1和p-Cofilin1表达。结果显示,DADS处理沉默组疤痕距离较对照组和沉默组增加(P<0.05)。DADS处理沉默组穿膜细胞低于对照组、空载体组与沉默组(P<0.05)。沉默组LIMK1表达低于对照组和空载体组,DADS后,各处理组低于处理前各组(P<0.05)。并且,沉默组、对照组和空载体组的Rac1、Rock1、Pak1与p-cofilin1表达下调(P<0.05)。表明DADS可增强沉默LIMK1抑制BGC823细胞迁移侵袭,机制可能与阻断Rac1/LIMK1/cofilin1通路有关。

To investigate the effect of diallyl disulfide(DADS)on the inhibition of BGC823 cell migration and invasion by silencing LIMK1 through Rac1/LIMK1/cofilin1 pathway.The ability of migration and invasion was observed by scratch experiment and invasion experiment.Expressions of LIMK1,Rac1,Rock1,Pak1 and Cofilin1 and p-cofilin1 were detected by RT-PCR,Western blot and immunohistochemistry.The results showed that the scar distance of DADS treated silent group increased compared with control group and silent group(P<0.05).The membrane penetrating cells in DADS treated silencing group were lower than those in control group,empty carrier group and silencing group(P<0.05).LIMK1 expression in the silent group was lower than that in the control group and the empty carrier group.After DADS,LIMK1 expression in the treatment group was lower than that in the control group and the empty carrier group(P<0.05).Moreover,the expressions of Rac1,Rock1,Pak1 and p-cofilin1 in the silent group,the control group and the empty carrier group were down-regulated(P<0.05).This suggested that DADS could enhance LIMK1 silencing and inhibit BGC823 cell migration and invasion,and the mechanism may be related to blocking the Rac1/LIMK1/cofilin1 pathway.