基于功能化N掺杂C3N4构建光电免疫传感器检测癌胚抗原

Bifunctional N-doped C3N4 for photoelectrochemical immunosensor of carcinoma embryonicantigen detection

分别合成掺杂不同比例N的C3N4纳米材料,利用红外光谱、荧光光谱等技术筛选出光电性能最优的N掺杂C3N4,并通过透射电镜、红外光谱等进行表征。将其作为光电转换材料,在氧化铟锡(ITO)电极表面构建高性能的免疫传感器。通过抗原-抗体的高特异性结合,实现对肿瘤标志物癌胚抗原的高灵敏检测。光电流结果表明:电流响应随癌胚抗原浓度的增加而减小,并在1.0 pg/mL^10 ng/mL的范围内呈现良好线性相关性。将此方法应用于检测血清样品中的癌胚抗原浓度,检测结果与商业化的酶联免疫分析方法结果进行对比没有显著差异。

N-doped C3N4 nanomaterials with different ratios of N were synthesized respectively.The N-doped C3N4 with the best photoelectric performance was screened by infrared spectroscopy and fluorescence spectroscopy,which was characterized by transmission electron microscopy and infrared spectroscopy.A high sensitive photoelectrochemical immunosensor was constructed based on N-doped C3N4 on the surface of ITO electrode for the detection of tumor marker carcinoembryonic antigen.Under the optimal experimental conditions,the photocurrent responses decreased with the increase of carcinoma embryonicantigen concentrations,and showed a good linear correlation in the range of 1.0 pg/m L-10 ng/m L.The immunosensor was applied to detect the concentration of carcinoembryonic antigen in real serum samples with no significant difference between the results of ELISA and commercial enzyme-linked immuno sorbent assay.