Nano-ZnO微叉指电极生物传感器检测大肠杆菌

Nano-ZnO micro-interdigitied electrode biosensor for detecting E.coli O157:H7

设计了一种基于纳米ZnO材料检测大肠杆菌(E.coli O157:H7)的微叉指阻抗生物传感器,利用电化学方法在氧化铟锡(ITO)叉指电极表面沉积上纳米ZnO,然后将链霉亲和素固定在纳米ZnO表面,利用生物素亲和素的高亲和性原理将大肠杆菌抗体绑定在传感器表面,完成传感器的构建。实验表明,传感器检测E.coli O157:H7线性范围为40~4×10^6cfu/mL,检出限为40 cfu/mL,传感器的特异性、重现性、实用性较好。

A nano-ZnO-based micro-interdigited impedance biosensor was designed for detecting E.coli O157:H7.Nano-ZnO was deposited on the surface of ITO interdigited electrode by electrochemical method.Then streptavidin was immobilized on the surface of nano-ZnO,the biotinized antibody was fixed on the surface of the sensor based on the high affinity principle between biotin and avidin,and the sensor construction was completed.The results showed that the linear detection range was 40 cfu/m L to 4×10^6 cfu/m L,and the detection limit of the sensor was 40 cfu/m L.The sensor also showed good specificity,reproducibility and practicability.