HPLC法测定云南白药瑶浴粉中盐酸水苏碱的含量

Determination of Stachydrine Hydrochloride in Yunnan Baiyao Yaoyu Powder by HPLC

建立云南白药瑶浴粉中有效含量检测的HPLC测定方法。试验与文献相结合,选出以盐酸水苏碱作为云南白药瑶浴粉含量检测的标志物成分之一,以树脂分离方法,提纯云南白药瑶浴粉中的盐酸水苏碱,以氨基硅烷键合硅胶为填充剂的色谱柱;乙腈-水(82∶18)为流动相;检测波长192 nm;柱温25℃的高效液相色谱方法。在进样量为2.4~24μg范围内,盐酸水苏碱对照品与峰面积呈良好的线性关系,r=0.9999,样品的平均回收率为99.9%。实验结果显示本方法专属性强,重现性好,可作为云南白药瑶浴浸膏粉中盐酸水苏碱的定量测定方法。

A HPLC method for the determination of the effective content of Yunnan Baiyao yaoyu powder was established.In combination with the literature,stachydrine hydrochloride was selected as one of the marker components for the determination of the content of Yunnan Baiyao yaoyu powder.By resin separation method,stachydrine hydrochloride in Yunnan Baiyao yaoyu powder was purified,and the chromatographic column with aminosilane bonded silica gel as the filler;acetonitrile water(82∶18)as the mobile phase;detection wavelength of 192 nm;column temperature of 25℃was used as the HPLC method.The calibration curve was linear over the range of 2.4~24μg of stachydrine hydrochloride(r=0.9999),the average recovery was 99.9%.The results showed that the method was specific and reproducible,and could be used for the quantitative determination of stachydrine hydrochloride in Yunnan Baiyao yaoyu extract powder.