标准化制片法在前列腺根治切除术标本病理诊断及Gleason评分中的应用价值

Application of standardized procedure of pathological sections of radical prostatectomy in pathological diagnosis and Gleason score

目的本研究旨在分析前列腺根治切除术标本HE制片流程中影响病理诊断以及Gleason评分的因素,探讨标准化制片流程在根治性前列腺癌病理诊断中的应用价值。方法回顾性收集2018-01—2018-05北京协和医院病理诊断为前列腺癌,并已明确Gleason分级的根治性前列腺切除术(radical prostatectomy,RP)23例,共1200张HE切片,选取医嘱为重切的HE切片共250张,全部使用全自动切片机采用标准化流程重新制片。比较两种制片流程,分别统计优片率和重切率。结果全自动切片机标准化流程制片法切面完整,厚薄适中,少刀痕、皱褶及颤痕;优片率高(99.2%),重切率低(0.8%)。结论全自动切片机标准化流程制作的HE切片优于手动切片机非标准化的HE切片,在根治性前列腺癌病理诊断中具有实际的应用价值,有助于根治性前列腺癌病理诊断及Gleason评分。

Objective To analyze the factors that affect pathological diagnosis and Gleason score in routine sections of radical prostatectomy tissues,so as to optimize the preparation process and further clarify the application value of standardized process sections in pathological diagnosis of radical prostate cancer.Methods Twenty-three patients,including 1,200 wax blocks,who received radical prostatectomy(RP)and diagnosed as prostate cancer with Gleason classification from January 2018 to May 2018,were retrospectively collected.Two hundred and fifty specimens included repeated slices.The 250 specimens were sliced with a fully automatic slicer following the standardized process section.The quality and re-cutting rates were compared and the rate of qualified slice and re-slice were calculated.Results The slices followed the standardized process section with complete section,moderate thickness,less knife marks,wrinkles and chatter marks.The rate of qualified slices was high(99.2%),while the re-cut rate was low(0.8%).Conclusion The routine section prepared by the standardized procedure is superior to non-standardized procedure which has practical application value in the pathological diagnosis of radical prostate cancer and Gleason score.